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. Author manuscript; available in PMC: 2013 Oct 10.
Published in final edited form as: Virology. 2012 Jun 28;432(1):120–126. doi: 10.1016/j.virol.2012.06.006

Figure 4. HPV16 E7 impedes the degradation of mitotic cyclins through SAC-dependent and SAC-independent mechanisms.

Figure 4

HFFs with stable expression of empty vector (C) or HPV16 E7 (E7) were transfected with non-targeting control siRNAs, siMad2, or siBubR1 before subjected to a double thymidine block. Western blot analyses of pRB, Mad2, BubR1, pH3S10, histone H3, cyclin B, cyclin A and actin are shown. Cyclin B and cyclin A quantifications relative to actin are indicated above the respective blots. Values are presented relative to the highest (1) in each condition. Shown here is a representative experiment; similar results were obtained in three additional experiments.