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. 2012 Jun 11;153(8):4030–4038. doi: 10.1210/en.2011-2113

Fig. 2.

Fig. 2.

A, Luciferase assay of Dio2-Luc promoter constructs in JEG3 cells. Cells were seeded in six-well plates and transfected with 1299 ATG, 805 ATG, 590 TSS, 120 TSS Dio2 Luc (schematic representation on the left), RSV β-gal, and C/EBPβ plasmids. At the end of the incubation, luciferase and β-galactosidase assays were performed. *, C/EBPβ vs. control, P < 0.05, Mann-Whitney test U test. Results represent the average value ± sd of three different experiments, each performed in triplicate. The numbered circles represent the potential Dio2 CCAAT elements. B, Luciferase assay of Dio2-Luc promoter constructs in JEG3 cells. Cells were seeded in six-well plates and transfected with 101 TSS, 81 TSS, 61 TSS, 41 TSS Dio2 Luc, RSV β gal and C/EBPβ plasmids. At the end of the incubation, luciferase and β-galactosidase assays were performed. *, C/EBPβ vs. control, P < 0.05, Mann-Whitney test U test. Results represent the average value ± sd of three different experiments, each performed in triplicate.