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. 1987 Feb 25;15(4):1363–1375. doi: 10.1093/nar/15.4.1363

Progress towards construction of a total restriction fragment map of a human chromosome.

H Vissing, F Grosveld, E Solomon, G Moore, N Lench, N Shennan, R Williamson
PMCID: PMC340554  PMID: 3029713

Abstract

We present an approach to the construction of an overlapping restriction fragment map of a single human chromosome. A genomic cosmid library genome was constructed from a mouse-human hybrid cell line containing chromosome 17 as its only human genetic component. Cosmids containing human inserts were isolated by hybridisation to a human Alu sequence. DNAs from ninety-six randomly chosen cosmids were digested with either EcoRI or HindIII, end-labelled with 35S-dATP and analysed using agarose gel electrophoresis. Comparison of the restriction fragment patterns revealed two pairs of overlapping clones, that were confirmed by cross-hybridization of the overlapping fragments. The two pairs of cosmids both mapped to human chromosome 17, as shown by hybridization to a panel of somatic cell hybrids. These data demonstrate that the generation of an overlapping cosmid map along a human chromosome is feasible, representing an intermediate step towards the complete sequencing of a human chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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