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. 1987 Feb 25;15(4):1717–1728. doi: 10.1093/nar/15.4.1717

Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia.

A Baroin, A Prat, F Caron
PMCID: PMC340576  PMID: 3029725

Abstract

In Paramecium primaurelia, the macronuclear gene encoding the G surface protein is located near a telomere. In this study, multiple copies of this telomere have been isolated and the subtelomeric and telomeric regions of some of them have been sequenced. The telomeric sequences consist of tandem repeats of the hexanucleotides C4A2 or C3A3. We show that the location where these repeats are added, which we call the telomeric site, is variable within a 0.6-0.8-kb region. These results are discussed in relation with the formation of macronuclear DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altschuler M. I., Yao M. C. Macronuclear DNA of Tetrahymena thermophila exists as defined subchromosomal-sized molecules. Nucleic Acids Res. 1985 Aug 26;13(16):5817–5831. doi: 10.1093/nar/13.16.5817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blackburn E. H., Budarf M. L., Challoner P. B., Cherry J. M., Howard E. A., Katzen A. L., Pan W. C., Ryan T. DNA termini in ciliate macronuclei. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 2):1195–1207. doi: 10.1101/sqb.1983.047.01.135. [DOI] [PubMed] [Google Scholar]
  3. Blackburn E. H., Gall J. G. A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena. J Mol Biol. 1978 Mar 25;120(1):33–53. doi: 10.1016/0022-2836(78)90294-2. [DOI] [PubMed] [Google Scholar]
  4. Capdeville Y. Allelic modulation in Paramecium aurelia heterozygotes. Study of G serotypes in syngen 1. Mol Gen Genet. 1971;112(4):306–316. doi: 10.1007/BF00334432. [DOI] [PubMed] [Google Scholar]
  5. Carle G. F., Olson M. V. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res. 1984 Jul 25;12(14):5647–5664. doi: 10.1093/nar/12.14.5647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Challoner P. B., Blackburn E. H. Conservation of sequences adjacent to the telomeric C4A2 repeats of ciliate macronuclear ribosomal RNA gene molecules. Nucleic Acids Res. 1986 Aug 11;14(15):6299–6311. doi: 10.1093/nar/14.15.6299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Engberg J., Andersson P., Leick V., Collins J. Free ribosomal DNA molecules from Tetrahymena pyriformis GL are giant palindromes. J Mol Biol. 1976 Jun 25;104(2):455–470. doi: 10.1016/0022-2836(76)90281-3. [DOI] [PubMed] [Google Scholar]
  8. Frischauf A. M., Lehrach H., Poustka A., Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. doi: 10.1016/s0022-2836(83)80190-9. [DOI] [PubMed] [Google Scholar]
  9. Gorovsky M. A. Genome organization and reorganization in Tetrahymena. Annu Rev Genet. 1980;14:203–239. doi: 10.1146/annurev.ge.14.120180.001223. [DOI] [PubMed] [Google Scholar]
  10. Greider C. W., Blackburn E. H. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell. 1985 Dec;43(2 Pt 1):405–413. doi: 10.1016/0092-8674(85)90170-9. [DOI] [PubMed] [Google Scholar]
  11. Karrer K. M., Gall J. G. The macronuclear ribosomal DNA of Tetrahymena pyriformis is a palindrome. J Mol Biol. 1976 Jun 25;104(2):421–453. doi: 10.1016/0022-2836(76)90280-1. [DOI] [PubMed] [Google Scholar]
  12. Katzen A. L., Cann G. M., Blackburn E. H. Sequence-specific fragmentation of macronuclear DNA in a holotrichous ciliate. Cell. 1981 May;24(2):313–320. doi: 10.1016/0092-8674(81)90321-4. [DOI] [PubMed] [Google Scholar]
  13. King B. O., Yao M. C. Tandemly repeated hexanucleotide at Tetrahymena rDNA free end is generated from a single copy during development. Cell. 1982 Nov;31(1):177–182. doi: 10.1016/0092-8674(82)90417-2. [DOI] [PubMed] [Google Scholar]
  14. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  15. McTavish C., Sommerville J. Macronuclear DNA organization and transcription in Paramecium primaurelia. Chromosoma. 1980;78(2):147–164. doi: 10.1007/BF00328389. [DOI] [PubMed] [Google Scholar]
  16. Meyer E., Caron F., Baroin A. Macronuclear structure of the G surface antigen gene of Paramecium primaurelia and direct expression of its repeated epitopes in Escherichia coli. Mol Cell Biol. 1985 Sep;5(9):2414–2422. doi: 10.1128/mcb.5.9.2414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pan W. C., Blackburn E. H. Single extrachromosomal ribosomal RNA gene copies are synthesized during amplification of the rDNA in Tetrahymena. Cell. 1981 Feb;23(2):459–466. doi: 10.1016/0092-8674(81)90141-0. [DOI] [PubMed] [Google Scholar]
  18. Prat A., Katinka M., Caron F., Meyer E. Nucleotide sequence of the Paramecium primaurelia G surface protein. A huge protein with a highly periodic structure. J Mol Biol. 1986 May 5;189(1):47–60. doi: 10.1016/0022-2836(86)90380-3. [DOI] [PubMed] [Google Scholar]
  19. Prescott D. M., Murti K. G. Chromosome structure in ciliated protozoans. Cold Spring Harb Symp Quant Biol. 1974;38:609–618. doi: 10.1101/sqb.1974.038.01.065. [DOI] [PubMed] [Google Scholar]
  20. Yao M. C., Gall J. G. A single integrated gene for ribosomal RNA in a eucaryote, Tetrahymena pyriformis. Cell. 1977 Sep;12(1):121–132. doi: 10.1016/0092-8674(77)90190-8. [DOI] [PubMed] [Google Scholar]
  21. Yao M. C. Ribosomal RNA gene amplification in Tetrahymena may be associated with chromosome breakage and DNA elimination. Cell. 1981 Jun;24(3):765–774. doi: 10.1016/0092-8674(81)90102-1. [DOI] [PubMed] [Google Scholar]
  22. Yao M. C., Yao C. H. Repeated hexanucleotide C-C-C-C-A-A is present near free ends of macronuclear DNA of Tetrahymena. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7436–7439. doi: 10.1073/pnas.78.12.7436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yao M. C., Zhu S. G., Yao C. H. Gene amplification in Tetrahymena thermophila: formation of extrachromosomal palindromic genes coding for rRNA. Mol Cell Biol. 1985 Jun;5(6):1260–1267. doi: 10.1128/mcb.5.6.1260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yokoyama R., Yao M. C. Sequence characterization of Tetrahymena macronuclear DNA ends. Nucleic Acids Res. 1986 Mar 11;14(5):2109–2122. doi: 10.1093/nar/14.5.2109. [DOI] [PMC free article] [PubMed] [Google Scholar]

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