Abstract
The major potato tuber protein, patatin, is a family of 40kd glycoproteins that constitutes forty per cent of the soluble protein in tubers but is generally undetectable in other tissues. Fused rocket immunoelectro-phoresis was used to detect in roots patatin that is immunologically different from tuber patatin. Western blots of SDS-polyacrylamide gels show root patatin to have a different molecular weight distribution than tuber patatin isoforms, but immunoprecipitation of in vitro translation products shows the patatin precursors to be of similar molecular weight in both tissues. This suggests that post-translational processing may differ in tubers and roots. Northern blots show that tuber and root patatin mRNAs are of similar size, but tuber transcripts are about 100-fold more abundant. 5' S1 nuclease and primer extension mapping suggests the class of patatin transcripts expressed in roots (class II transcripts) to be a subset of patatin transcripts expressed in tubers (classes I and II). Class II patatin mRNAs differ from class I transcripts by the presence of a 22 nucleotide insertion just upstream of the initiation codon. These data demonstrate that expression of the patatin multigene family is differentially regulated in tubers and roots.
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Selected References
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- Bailey J. M., Davidson N. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976 Jan;70(1):75–85. doi: 10.1016/s0003-2697(76)80049-8. [DOI] [PubMed] [Google Scholar]
- Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
- Bevan M., Barker R., Goldsbrough A., Jarvis M., Kavanagh T., Iturriaga G. The structure and transcription start site of a major potato tuber protein gene. Nucleic Acids Res. 1986 Jun 11;14(11):4625–4638. doi: 10.1093/nar/14.11.4625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
- Ghosh P. K., Reddy V. B., Swinscoe J., Lebowitz P., Weissman S. M. Heterogeneity and 5'-terminal structures of the late RNAs of simian virus 40. J Mol Biol. 1978 Dec 25;126(4):813–846. doi: 10.1016/0022-2836(78)90022-0. [DOI] [PubMed] [Google Scholar]
- Hasson E. P., Laties G. G. Purification and characterization of an a type phospholipase from potato and its effect on potato mitochondria. Plant Physiol. 1976 Feb;57(2):148–152. doi: 10.1104/pp.57.2.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Mignery G. A., Pikaard C. S., Hannapel D. J., Park W. D. Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatin. Nucleic Acids Res. 1984 Nov 12;12(21):7987–8000. doi: 10.1093/nar/12.21.7987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paiva E., Lister R. M., Park W. D. Induction and accumulation of major tuber proteins of potato in stems and petioles. Plant Physiol. 1983 Jan;71(1):161–168. doi: 10.1104/pp.71.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Park W. D., Blackwood C., Mignery G. A., Hermodson M. A., Lister R. M. Analysis of the Heterogeneity of the 40,000 Molecular Weight Tuber Glycoprotein of Potatoes by Immunological Methods and by NH(2)-Terminal Sequence Analysis. Plant Physiol. 1983 Jan;71(1):156–160. doi: 10.1104/pp.71.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pikaard C. S., Mignery G. A., Ma D. P., Stark V. J., Park W. D. Sequence of two apparent pseudogenes of the major potato tuber protein, patatin. Nucleic Acids Res. 1986 Jul 11;14(13):5564–5566. doi: 10.1093/nar/14.13.5564. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]