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. 1987 Mar 25;15(6):2403–2416. doi: 10.1093/nar/15.6.2403

Properties of a U1 RNA enhancer-like sequence.

G Ciliberto, F Palla, G Tebb, I W Mattaj, L Philipson
PMCID: PMC340659  PMID: 3031597

Abstract

The properties of a X.laevis U1B snRNA gene enhancer have been studied by microinjection in Xenopus oocytes. The enhancer-like sequence, defined as a short DNA stretch that is able to activate transcription in an orientation independent manner, is interchangeable between different U snRNA genes. The enhancer sequence alone does not, however, efficiently activate transcription from an SV40 pol II promoter but regains its activity when combined with the U-gene specific proximal sequence element. DNase I protection experiments show that the X.laevis U1B enhancer can interact specifically with a nuclear factor present in mammalian cells.

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Selected References

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