Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1987 Apr 24;15(8):3305–3315. doi: 10.1093/nar/15.8.3305

All foot and mouth disease virus serotypes initiate protein synthesis at two separate AUGs.

D V Sangar, S E Newton, D J Rowlands, B E Clarke
PMCID: PMC340731  PMID: 3033601

Abstract

Translation of the foot and mouth disease virus genome in vitro and in vivo indicated that all seven serotypes initiate protein synthesis at two separate AUGs. Sequence analysis of the region surrounding these AUGs has shown that the efficiency with which the initiating AUG is recognized is dependent on the flanking nucleotides. However, in vitro, the major factor determining which AUG is used is the concentration of Mg2+.

Full text

PDF
3305

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beck E., Forss S., Strebel K., Cattaneo R., Feil G. Structure of the FMDV translation initiation site and of the structural proteins. Nucleic Acids Res. 1983 Nov 25;11(22):7873–7885. doi: 10.1093/nar/11.22.7873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Caton A. J., Brownlee G. G., Yewdell J. W., Gerhard W. The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype). Cell. 1982 Dec;31(2 Pt 1):417–427. doi: 10.1016/0092-8674(82)90135-0. [DOI] [PubMed] [Google Scholar]
  3. Clarke B. E., Sangar D. V., Burroughs J. N., Newton S. E., Carroll A. R., Rowlands D. J. Two initiation sites for foot-and-mouth disease virus polyprotein in vivo. J Gen Virol. 1985 Dec;66(Pt 12):2615–2626. doi: 10.1099/0022-1317-66-12-2615. [DOI] [PubMed] [Google Scholar]
  4. Dorner A. J., Dorner L. F., Larsen G. R., Wimmer E., Anderson C. W. Identification of the initiation site of poliovirus polyprotein synthesis. J Virol. 1982 Jun;42(3):1017–1028. doi: 10.1128/jvi.42.3.1017-1028.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Forss S., Strebel K., Beck E., Schaller H. Nucleotide sequence and genome organization of foot-and-mouth disease virus. Nucleic Acids Res. 1984 Aug 24;12(16):6587–6601. doi: 10.1093/nar/12.16.6587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kozak M. Evaluation of the "scanning model" for initiation of protein synthesis in eucaryotes. Cell. 1980 Nov;22(1 Pt 1):7–8. doi: 10.1016/0092-8674(80)90148-8. [DOI] [PubMed] [Google Scholar]
  7. Kozak M. Influences of mRNA secondary structure on initiation by eukaryotic ribosomes. Proc Natl Acad Sci U S A. 1986 May;83(9):2850–2854. doi: 10.1073/pnas.83.9.2850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986 Jan 31;44(2):283–292. doi: 10.1016/0092-8674(86)90762-2. [DOI] [PubMed] [Google Scholar]
  9. Kozak M. Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin. Nucleic Acids Res. 1984 May 11;12(9):3873–3893. doi: 10.1093/nar/12.9.3873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Newton S. E., Carroll A. R., Campbell R. O., Clarke B. E., Rowlands D. J. The sequence of foot-and-mouth disease virus RNA to the 5' side of the poly(C) tract. Gene. 1985;40(2-3):331–336. doi: 10.1016/0378-1119(85)90057-5. [DOI] [PubMed] [Google Scholar]
  12. Pelham H. R., Jackson R. J. An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem. 1976 Aug 1;67(1):247–256. doi: 10.1111/j.1432-1033.1976.tb10656.x. [DOI] [PubMed] [Google Scholar]
  13. Rowlands D. J., Harris T. J., Brown F. More precise location of the polycytidylic acid tract in foot and mouth disease virus RNA. J Virol. 1978 May;26(2):335–343. doi: 10.1128/jvi.26.2.335-343.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sangar D. V., Black D. N., Rowlands D. J., Harris T. J., Brown F. Location of the initiation site for protein synthesis on foot-and-mouth disease virus RNA by in vitro translation of defined fragments of the RNA. J Virol. 1980 Jan;33(1):59–68. doi: 10.1128/jvi.33.1.59-68.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sangar D. V. The replication of picornaviruses. J Gen Virol. 1979 Oct;45(1):1–13. doi: 10.1099/0022-1317-45-1-1. [DOI] [PubMed] [Google Scholar]
  16. Strebel K., Beck E. A second protease of foot-and-mouth disease virus. J Virol. 1986 Jun;58(3):893–899. doi: 10.1128/jvi.58.3.893-899.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES