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. 1987 Apr 24;15(8):3549–3561. doi: 10.1093/nar/15.8.3549

Cell cycle regulated synthesis of an abundant transcript for human chromosomal protein HMG-17.

M Bustin, N Soares, D Landsman, T Srikantha, J M Collins
PMCID: PMC340748  PMID: 3575100

Abstract

The abundance and cell cycle dependent expression of the mRNA for human nonhistone protein HMG-17 were studied in synchronized HeLa cells. Slot blot analysis indicates that the HMG-17 mRNA is a very abundant message, significantly more so than histone or actin mRNA. RNA prepared from tissue culture cells contains higher amounts of HMG-17 transcripts than RNA prepared from liver suggesting a correlation between the rate of cell division and HMG-17 mRNA levels. HMG-17 mRNA is present in the cells throughout the cell cycle however there is a significant increase in the mRNA levels late in S phase suggesting that the protein is deposited on chromatin after nucleosome assembly. Synthesis of the HMG-17 transcript is not coupled to DNA replication suggesting that the cell cycle related expression during late S phase is regulated in a different manner from that of the nucleosomal histones.

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Selected References

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