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. 1987 May 11;15(9):3835–3843. doi: 10.1093/nar/15.9.3835

Supercoiling-dependent sequence specificity of mammalian DNA methyltransferase.

T Bestor
PMCID: PMC340785  PMID: 3473446

Abstract

Negative supercoiling of substrate DNA dramatically alters the in vitro sequence specificity of mammalian DNA methyltransferase (DNA MeTase). This result suggests that in vivo site selection by DNA MeTase could be regulated by conformational information in the form of alternative secondary structures induced in DNA by local supercoiling or by the binding of specific nuclear proteins. DNA in the left-handed Z-form is shown not to be a substrate for mammalian DNA MeTase. The sensitivity of DNA MeTase to DNA structure may also make it useful as a probe for sequences which undergo supercoiling-dependent structural transitions in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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