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. 1987 Jun 11;15(11):4513–4534. doi: 10.1093/nar/15.11.4513

Novel biotinylated nucleotide--analogs for labeling and colorimetric detection of DNA.

G Gebeyehu, P Y Rao, P SooChan, D A Simms, L Klevan
PMCID: PMC340877  PMID: 3588302

Abstract

A series of dATP and dCTP nucleotide analogs have been synthesized which are modified by attachment of aliphatic linkers containing a functional group to the amino-nitrogen at the hydrogen bonding positions of the bases, that is, at the 6-position of adenine and the 4-position of cytosine. These nucleotides are incorporated into DNA probes by standard nick-translation protocols. DNA probes labeled with biotin derivatives of these nucleotides are effectively hybridized to target DNA sequences and can be detected by a streptavidin and calf intestinal alkaline phosphatase conjugate with a sensitivity (0.25 pg DNA) sufficient for reproducible and rapid detection of single copy genes in a Southern blot of mammalian DNA. Also, a procedure has been developed to allow reprobing of nylon filters that have been hybridized with biotinylated probes and developed with the streptavidin/alkaline phosphatase conjugate and a standard dye system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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