Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1987 Jun 11;15(11):4583–4591. doi: 10.1093/nar/15.11.4583

The sequence of the 6S RNA gene of Pseudomonas aeruginosa.

D W Vogel, R K Hartmann, J C Struck, N Ulbrich, V A Erdmann
PMCID: PMC340881  PMID: 2438656

Abstract

From the gram-negative eubacterium Pseudomonas aeruginosa we have isolated a stable 6S RNA, approximately 180 nucleotides in length. The RNA was partially sequenced and identified by comparison with the known Escherichia coli 6S RNA sequence. Southern hybridizations revealed a single copy gene coding for the 6S RNA. DNA from other prokaryotes, i.e. E. coli, Thermus thermophilus, Bacillus subtilis, Bacillus stearothermophilus and Halobacterium maris mortui, did not give detectable hybridization signals. The 6S RNA gene was cloned in E. coli and its complete primary structure was determined. Although the 6S RNA sequences from P. aeruginosa and E. coli share only a 60.4% homology, we are able to propose a common secondary structural model.

Full text

PDF
4583

Images in this article

4586Image
on p.4586

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brownlee G. G. Sequence of 6S RNA of E. coli. Nat New Biol. 1971 Feb 3;229(5):147–149. doi: 10.1038/newbio229147a0. [DOI] [PubMed] [Google Scholar]
  3. Christiansen J., Douthwaite S. R., Christensen A., Garrett R. A. Does unpaired adenosine-66 from helix II of Escherichia coli 5S RNA bind to protein L18? EMBO J. 1985 Apr;4(4):1019–1024. doi: 10.1002/j.1460-2075.1985.tb03733.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Digweed M., Pieler T., Kluwe D., Schuster L., Walker R., Erdmann V. A. Improved procedure for the isolation of a double-strand-specific ribonuclease and its application to structural analysis of various 5S rRNAs and tRNAs. Eur J Biochem. 1986 Jan 2;154(1):31–39. doi: 10.1111/j.1432-1033.1986.tb09355.x. [DOI] [PubMed] [Google Scholar]
  5. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. England T. E., Uhlenbeck O. C. 3'-terminal labelling of RNA with T4 RNA ligase. Nature. 1978 Oct 12;275(5680):560–561. doi: 10.1038/275560a0. [DOI] [PubMed] [Google Scholar]
  7. Gardiner K., Pace N. R. RNase P of Bacillus subtilis has a RNA component. J Biol Chem. 1980 Aug 25;255(16):7507–7509. [PubMed] [Google Scholar]
  8. Griffin B. E., Baillie D. L. Precursors of stable RNA accumulated in a mutant of E. coli. FEBS Lett. 1973 Aug 15;34(2):273–279. doi: 10.1016/0014-5793(73)80811-7. [DOI] [PubMed] [Google Scholar]
  9. Griffin B. E. Studies and sequences of Escherichia coli 4.5 S RNA. J Biol Chem. 1975 Jul 25;250(14):5426–5437. [PubMed] [Google Scholar]
  10. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  11. Hartmann R. K., Toschka H. Y., Ulbrich N., Erdmann V. A. Genomic organization of rDNA in Pseudomonas aeruginosa. FEBS Lett. 1986 Jan 20;195(1-2):187–193. doi: 10.1016/0014-5793(86)80158-2. [DOI] [PubMed] [Google Scholar]
  12. Hawley D. K., McClure W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983 Apr 25;11(8):2237–2255. doi: 10.1093/nar/11.8.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Horne J. R., Erdmann V. A. Isolation and characterization of 5S RNA-protein complexes from Bacillus stearothermophilus and Escherichia coli ribosomes. Mol Gen Genet. 1972;119(4):337–344. doi: 10.1007/BF00272091. [DOI] [PubMed] [Google Scholar]
  14. Hsu L. M., Zagorski J., Wang Z., Fournier M. J. Escherichia coli 6S RNA gene is part of a dual-function transcription unit. J Bacteriol. 1985 Mar;161(3):1162–1170. doi: 10.1128/jb.161.3.1162-1170.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ikemura T., Dahlberg J. E. Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis. J Biol Chem. 1973 Jul 25;248(14):5024–5032. [PubMed] [Google Scholar]
  16. Ikemura T., Dahlberg J. E. Small ribonucleic acids of Escherichia coli. II. Noncoordinate accumulation during stringent control. J Biol Chem. 1973 Jul 25;248(14):5033–5041. [PubMed] [Google Scholar]
  17. Lee C. A., Fournier M. J., Beckwith J. Escherichia coli 6S RNA is not essential for growth or protein secretion. J Bacteriol. 1985 Mar;161(3):1156–1161. doi: 10.1128/jb.161.3.1156-1161.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lee S. Y., Bailey S. C., Apirion D. Small stable RNAs from Escherichia coli: evidence for the existence of new molecules and for a new ribonucleoprotein particle containing 6S RNA. J Bacteriol. 1978 Feb;133(2):1015–1023. doi: 10.1128/jb.133.2.1015-1023.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Müller M., Blobel G. Protein export in Escherichia coli requires a soluble activity. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7737–7741. doi: 10.1073/pnas.81.24.7737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Reich C., Gardiner K. J., Olsen G. J., Pace B., Marsh T. L., Pace N. R. The RNA component of the Bacillus subtilis RNase P. Sequence, activity, and partial secondary structure. J Biol Chem. 1986 Jun 15;261(17):7888–7893. [PubMed] [Google Scholar]
  21. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  22. Robertson M. Membrane traffic and the problem of protein secretion. Nature. 1984 Feb 16;307(5952):594–595. doi: 10.1038/307594a0. [DOI] [PubMed] [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  25. Timmis K. N., Cabello F., Cohen S. N. Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Mol Gen Genet. 1978 Jun 14;162(2):121–137. doi: 10.1007/BF00267869. [DOI] [PubMed] [Google Scholar]
  26. Travers A. A. Conserved features of coordinately regulated E. coli promoters. Nucleic Acids Res. 1984 Mar 26;12(6):2605–2618. doi: 10.1093/nar/12.6.2605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Ulbrich N., Wool I. G. Identification by affinity chromatography of the eukaryotic ribosomal proteins that bind to 5 S ribosomal ribonucleic acid. J Biol Chem. 1978 Dec 25;253(24):9049–9052. [PubMed] [Google Scholar]
  28. Walter P., Blobel G. Disassembly and reconstitution of signal recognition particle. Cell. 1983 Sep;34(2):525–533. doi: 10.1016/0092-8674(83)90385-9. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES