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. 1985 Jan 11;13(1):131–140. doi: 10.1093/nar/13.1.131

Complete nucleotide sequence of the fumarase gene (citG) of Bacillus subtilis 168.

J S Miles, J R Guest
PMCID: PMC340979  PMID: 3923430

Abstract

The nucleotide sequence of a 2.14 kb fragment of Bacillus subtilis DNA containing the citG gene encoding fumarase was determined using the dideoxy chain termination method. The citG coding region of 1392 base pairs (464 codons) was identified, and the deduced Mr (50425) is in good agreement with that of the protein identified from expression in Escherichia coli maxicells. There is no sequence homology between the B. subtilis and E. coli fumarases. Overlapping potential promoter sequences have been identified for sigma 28, sigma 37 and sigma 55 RNA polymerase holoenzymes. The DNA fragment also contains the proximal part of the gerA locus, responsible for L-alanine-sensitive spore germination.

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Selected References

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