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. 2012 Aug;97(8):1264–1271. doi: 10.3324/haematol.2011.051409

Figure 2.

Figure 2.

PolyP in primary MC. (A) Confocal fluorescence microscopy of fixed human myeloma cells after specific polyP labeling with DAPI. Cells were isolated, by CD138-dependent immunomagnetic selection, from the bone marrow of six patients with multiple myeloma (MM). Bar: 10 μm. Representative pictures are shown (patient 1 and patient 2). (B) Flow cytometry analysis of isolated bone marrow mononuclear cells, from MM patients. Fixed cells, labeled with anti-CD138 phycoerythrin antibodies, were incubated in the absence (Control) and presence of DAPI (+DAPI), as described in the legend of Figure 1. The geometric mean fluorescence intensities (MFI), in the FL3 channel of the flow cytometer, are indicated for the MC (CD 138+). Measurements for one representative sample of six are shown. (C) Quantification of the differences in the geometric MFI (ΔMFI), in the FL3 channel, after the addition of DAPI. MC (CD 138+) and other mononuclear cells (CD138-) from bone marrow samples were analyzed as described in panel (B). Results represent the mean ± SD of the measurements made in the samples of six MM patients. The asterisk indicates a significant difference in comparison with primary MC, as determined by the t test (P<0.05).