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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Parasitology. 2007 Jul 5;134(Pt 12):1679–1687. doi: 10.1017/S0031182007003150

Figure 6. Flow cytometric detection of ROS scavenging activity in Sb-S and Sb-R strains.

Figure 6

Log phase promastigotes from Sb-S strains S1 (closed squares, -■-), S2 (open squares, -□-) and Sb-R strains R1 (inverted closed triangles, -▼-), R2 (upright open triangles, -Δ-) and R3 (open circles, -○-) were exposed to increasing concentration of H2O2 (0 - 1000 μM) at 37°C for 1 h, probed with dichlorodihydrofluorescein diacetate (H2DCFDA) at 37°C for 45 for minutes and dichlorofluorescein (DCF) fluorescence was analysed as described in Materials and Methods. Data were expressed as MFC ± SEM of at least three independent experiments in duplicates.