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. 1985 Feb 25;13(4):1341–1345. doi: 10.1093/nar/13.4.1341

Yeast mitochondrial tRNAIle and tRNAMetm: nucleotide sequence and codon recognition patterns.

A P Sibler, G Dirheimer, R P Martin
PMCID: PMC341076  PMID: 3889839

Abstract

The nucleotide sequence of yeast mitochondrial isoleucine- and methionine-elongator tRNA have been determined. Interestingly, long stretches of almost identical nucleotide sequences are found within these two tRNAs and also within the yeast mt tRNAMetf, suggesting that the 3 tRNAs may have arisen from a common ancestor. Both mt tRNAMetm and tRNAIle contain all the structural characteristics which are present in the standard cloverleaf, except that the mt tRNAMetm contains an extra unpaired nucleotide within the base-paired T psi C stem. This rather unusual feature may have an influence on the decoding properties of the C-A-U anticodon of mt tRNAMetm by conferring the ability to translate not only the codon A-U-G but also A-U-A.

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Selected References

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  1. Anderson S., Bankier A. T., Barrell B. G., de Bruijn M. H., Coulson A. R., Drouin J., Eperon I. C., Nierlich D. P., Roe B. A., Sanger F. Sequence and organization of the human mitochondrial genome. Nature. 1981 Apr 9;290(5806):457–465. doi: 10.1038/290457a0. [DOI] [PubMed] [Google Scholar]
  2. Bonitz S. G., Tzagoloff A. Assembly of the mitochondrial membrane system. Sequences of yeast mitochondrial tRNA genes. J Biol Chem. 1980 Oct 10;255(19):9075–9081. [PubMed] [Google Scholar]
  3. Browning K. S., RajBhandary U. L. Cytochrome oxidase subunit III gene in Neurospora crassa mitochondria. Location and sequence. J Biol Chem. 1982 May 10;257(9):5253–5256. [PubMed] [Google Scholar]
  4. Canaday J., Dirheimer G., Martin R. P. Yeast mitochondrial methionine initiator tRNA: characterization and nucleotide sequence. Nucleic Acids Res. 1980 Apr 11;8(7):1445–1457. doi: 10.1093/nar/8.7.1445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coruzzi G., Bonitz S. G., Thalenfeld B. E., Tzagoloff A. Assembly of the mitochondrial membrane system. Analysis of the nucleotide sequence and transcripts in the oxi1 region of yeast mitochondrial DNA. J Biol Chem. 1981 Dec 25;256(24):12780–12787. [PubMed] [Google Scholar]
  6. Diamond A., Dudock B., Hatfield D. Structure and properties of a bovine liver UGA suppressor serine tRNA with a tryptophan anticodon. Cell. 1981 Aug;25(2):497–506. doi: 10.1016/0092-8674(81)90068-4. [DOI] [PubMed] [Google Scholar]
  7. Francis M. A., Dudock B. S. Nucleotide sequence of a spinach chloroplast isoleucine tRNA. J Biol Chem. 1982 Oct 10;257(19):11195–11198. [PubMed] [Google Scholar]
  8. Fukada K., Abelson J. DNA sequence of a T4 transfer RNA gene cluster. J Mol Biol. 1980 May 25;139(3):377–391. doi: 10.1016/0022-2836(80)90136-9. [DOI] [PubMed] [Google Scholar]
  9. Hirsh D. Tryptophan transfer RNA as the UGA suppressor. J Mol Biol. 1971 Jun 14;58(2):439–458. doi: 10.1016/0022-2836(71)90362-7. [DOI] [PubMed] [Google Scholar]
  10. Hudspeth M. E., Ainley W. M., Shumard D. S., Butow R. A., Grossman L. I. Location and structure of the var1 gene on yeast mitochondrial DNA: nucleotide sequence of the 40.0 allele. Cell. 1982 Sep;30(2):617–626. doi: 10.1016/0092-8674(82)90258-6. [DOI] [PubMed] [Google Scholar]
  11. Kuchino Y., Watanabe S., Harada F., Nishimura S. Primary structure of AUA-specific isoleucine transfer ribonucleic acid from Escherichia coli. Biochemistry. 1980 May 13;19(10):2085–2089. doi: 10.1021/bi00551a013. [DOI] [PubMed] [Google Scholar]
  12. Martin R. P., Schneller J. M., Stahl A. J., Dirheimer G. Study of yeast mitochondrial tRNAs by two-dimensional polyacrylamide gel electrophoresis: characterization of isoaccepting species and search for imported cytoplasmic tRNAs. Nucleic Acids Res. 1977 Oct;4(10):3497–3510. doi: 10.1093/nar/4.10.3497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Martin R. P., Sibler A. P., Schneller J. M., Keith G., Stahl A. J., Dirheimer G. Primary structure of yeast mitochondrial DNA-coded phenylalanine-tRNA. Nucleic Acids Res. 1978 Dec;5(12):4579–4592. doi: 10.1093/nar/5.12.4579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Martin R., Sibler A. P., Dirheimer G. The primary structures of three yeast mitochondrial serine tRNA isoacceptors. Biochimie. 1982 Nov-Dec;64(11-12):1073–1079. doi: 10.1016/s0300-9084(82)80389-1. [DOI] [PubMed] [Google Scholar]
  15. Mikelsaar R. C-A base pairs in transfer ribonucleic acids and codon-anticodon complexes. J Theor Biol. 1981 Sep 21;92(2):163–180. doi: 10.1016/0022-5193(81)90390-8. [DOI] [PubMed] [Google Scholar]
  16. Sibler A. P., Martin R. P., Dirheimer G. The nucleotide sequence of yeast mitochondrial histidine-tRNA. FEBS Lett. 1979 Nov 1;107(1):182–186. doi: 10.1016/0014-5793(79)80491-3. [DOI] [PubMed] [Google Scholar]
  17. Stanley J., Vassilenko S. A different approach to RNA sequencing. Nature. 1978 Jul 6;274(5666):87–89. doi: 10.1038/274087a0. [DOI] [PubMed] [Google Scholar]
  18. Waring R. B., Davies R. W., Lee S., Grisi E., Berks M. M., Scazzocchio C. The mosaic organization of the apocytochrome b gene of Aspergillus nidulans revealed by DNA sequencing. Cell. 1981 Nov;27(1 Pt 2):4–11. doi: 10.1016/0092-8674(81)90354-8. [DOI] [PubMed] [Google Scholar]

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