Table III.

Level of DNA strand breaks for females, dams or offspring exposed to Printex 90 by inhalation or intratracheal instillation and control mice.

	Inhalation		Instillation
	Control	Printex 90 41.7 mg/m3 ∼ 287 mg/animal	Control	Printex 90 11 mg/animal	Printex 90 54 mg/animal	Printex 90 268 mg/animal
Females 3–5 days after exposure
BAL	0.55 ± 0.06	0.70 ± 0.10	0.90 ± 0.04	0.91 ± 0.09	0.78 ± 0.07	0.72 ± 0.07
Liver	2.57 ± 0.19	3.25 ± 0.21*	0.66 ± 0.06	0.55 ± 0.07	0.57 ± 0.07	0.52 ± 0.07
Dams at weaning 24–27 days after exposure
BAL	0.58 ± 0.05	0.60 ± 0.04	0.62 ± 0.02	0.69 ± 0.06	0.58 ± 0.01	0.50 ± 0.03**
Liver	1.24 ± 0.07	1.94 ± 0.11***	0.52 ± 0.05	0.55 ± 0.09	0.48 ± 0.02	0.58 ± 0.04
Offspring
Liver newborns PND 2	3.50 ± 0.28	3.64 ± 0.37	0.66 ± 0.02	0.65 ± 0.03	0.73 ± 0.02	0.64 ± 0.03
Liver offspring at weaning PND 22–23	1.13 ± 0.09	1.56 ± 0.08***	0.39 ± 0.03	0.41 ± 0.04	0.42 ± 0.04	0.42 ± 0.03
Liver adolescents PND 50 (47)	1.10 ± 0.16	1.69 ± 0.14**	0.54 ± 0.04	0.48 ± 0.04	0.47 ± 0.04	0.52 ± 0.03

BAL, bronchoalveolar lavage; PND, post natal day (days after birth). Data are presented as mean number of lesions per 106 base pairs ± SEM (calculated from %DNA results). Offspring data are calculated as litter average, when sibling liver tissues were analyzed at the same collection point.

^*p < 0.05,

^**p < 0.01,

^***p < 0.001.