Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1985 Mar 25;13(6):1855–1869. doi: 10.1093/nar/13.6.1855

The linear extrachromosomal DNA of Physarum polycephalum replicates and is maintained under non-selective conditions in two different lower eukaryotes.

P Künzler
PMCID: PMC341121  PMID: 3889843

Abstract

The slime mould Physarum polycephalum contains 100 to 200 molecules of extrachromosomal linear DNA (PeDNA). Two sets of the 19S and 26S ribosomal genes are located on each molecule of PeDNA. In the nonmitotic phase of the cell cycle PeDNA is localised in the nucleolus. The molecules are maintained throughout vegetative growth. In order to study the signals responsible for its maintenance, PeDNA was purified and introduced into the two distantly related yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. Surprisingly, intact PeDNA transforms both yeasts with high frequency and PeDNA sequences are maintained in the absence of selective pressure.

Full text

PDF
1855

Images in this article

1859Image
on p.1859
1861Image
on p.1861
1862Image
on p.1862
1863Image
on p.1863
1864Image
on p.1864

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beach D., Piper M., Nurse P. Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation. Mol Gen Genet. 1982;187(2):326–329. doi: 10.1007/BF00331138. [DOI] [PubMed] [Google Scholar]
  2. Blackburn E. H. Telomeres: do the ends justify the means? Cell. 1984 May;37(1):7–8. doi: 10.1016/0092-8674(84)90295-2. [DOI] [PubMed] [Google Scholar]
  3. Bloom K. S., Carbon J. Yeast centromere DNA is in a unique and highly ordered structure in chromosomes and small circular minichromosomes. Cell. 1982 Jun;29(2):305–317. doi: 10.1016/0092-8674(82)90147-7. [DOI] [PubMed] [Google Scholar]
  4. Clarke L., Carbon J. Isolation of a yeast centromere and construction of functional small circular chromosomes. Nature. 1980 Oct 9;287(5782):504–509. doi: 10.1038/287504a0. [DOI] [PubMed] [Google Scholar]
  5. Dani G. M., Zakian V. A. Mitotic and meiotic stability of linear plasmids in yeast. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3406–3410. doi: 10.1073/pnas.80.11.3406. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dawson D., Herrick G. Telomeric properties of C4A4-homologous sequences in micronuclear DNA of Oxytricha fallax. Cell. 1984 Jan;36(1):171–177. doi: 10.1016/0092-8674(84)90086-2. [DOI] [PubMed] [Google Scholar]
  7. Egel R., Kohli J., Thuriaux P., Wolf K. Genetics of the fission yeast Schizosaccharomyces pombe. Annu Rev Genet. 1980;14:77–108. doi: 10.1146/annurev.ge.14.120180.000453. [DOI] [PubMed] [Google Scholar]
  8. Ferris P. J., Vogt V. M. Structure of the central spacer region of extrachromosomal ribosomal DNA in Physarum polycephalum. J Mol Biol. 1982 Aug 15;159(3):359–381. doi: 10.1016/0022-2836(82)90289-3. [DOI] [PubMed] [Google Scholar]
  9. Hattori M., Ljljana A., Sakaki Y. Direct repeats surrounding the ribosomal RNA genes of Physarum polycephalum. Nucleic Acids Res. 1984 Feb 24;12(4):2047–2053. doi: 10.1093/nar/12.4.2047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hinnen A., Hicks J. B., Fink G. R. Transformation of yeast. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929–1933. doi: 10.1073/pnas.75.4.1929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johnson E. M. A family of inverted repeat sequences and specific single-strand gaps at the termini of the Physarum rDNA palindrome. Cell. 1980 Dec;22(3):875–886. doi: 10.1016/0092-8674(80)90564-4. [DOI] [PubMed] [Google Scholar]
  12. Kiss G. B., Amin A. A., Pearlman R. E. Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae. Mol Cell Biol. 1981 Jun;1(6):535–543. doi: 10.1128/mcb.1.6.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Long E. O., Dawid I. B. Repeated genes in eukaryotes. Annu Rev Biochem. 1980;49:727–764. doi: 10.1146/annurev.bi.49.070180.003455. [DOI] [PubMed] [Google Scholar]
  14. Otsuka T., Nomiyama H., Yoshida H., Kukita T., Kuhara S., Sakaki Y. Complete nucleotide sequence of the 26S rRNA gene of Physarum polycephalum: its significance in gene evolution. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3163–3167. doi: 10.1073/pnas.80.11.3163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pauli U. H., Seebeck T., Braun R. Sequence-specific cleavage of chromatin by staphylococcal nuclease can generate an atypical nucleosome pattern. Nucleic Acids Res. 1982 Jul 24;10(14):4121–4133. doi: 10.1093/nar/10.14.4121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stinchcomb D. T., Struhl K., Davis R. W. Isolation and characterisation of a yeast chromosomal replicator. Nature. 1979 Nov 1;282(5734):39–43. doi: 10.1038/282039a0. [DOI] [PubMed] [Google Scholar]
  17. Szostak J. W., Blackburn E. H. Cloning yeast telomeres on linear plasmid vectors. Cell. 1982 May;29(1):245–255. doi: 10.1016/0092-8674(82)90109-x. [DOI] [PubMed] [Google Scholar]
  18. Tschumper G., Carbon J. Copy number control by a yeast centromere. Gene. 1983 Aug;23(2):221–232. doi: 10.1016/0378-1119(83)90054-9. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES