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. 1985 Mar 25;13(6):1923–1938. doi: 10.1093/nar/13.6.1923

Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I).

G Buell, M F Schulz, G Selzer, A Chollet, N R Movva, D Semon, S Escanez, E Kawashima
PMCID: PMC341125  PMID: 3889844

Abstract

Double-stranded DNA encoding the human hormone somatomedin-C (SMC) has been synthesized. This synthetic gene has been inserted into a plasmid bearing the strong leftward promoter (PL) of bacteriophage lambda and expressed in E. coli. Codons for the N-terminal region of SMC which maximized the hormone's synthesis were chosen in an SMC-lac z fusion assay. The amounts of SMC accumulated in E. coli were influenced by mutations at two chromosomal loci, lon and htpR.

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Selected References

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