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. 1985 Apr 11;13(7):2519–2531. doi: 10.1093/nar/13.7.2519

Analysis of the promoters for the two immunity genes present in the ColE3-CA38 plasmid using two new promoter probe vectors.

K F Chak, R James
PMCID: PMC341172  PMID: 2987857

Abstract

We have constructed two new promoter probe vectors which carry a polylinker derived from plasmid pUC19 proximal to the 5' end of a promoter-less galactokinase gene. Using these two vectors we have demonstrated that the ColE3imm gene and the ColE8imm gene present on the ColE3-CA38 plasmid have their own promoters, independent of the SOS promoter of the colicin E3 structural gene. The activity of two terminators, one located proximal to the 5' end of the ColE8imm gene, the other located proximal to the 5' end of the lys gene, were shown by a comparison of the galactokinase activity conferred by several of the recombinant plasmids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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