Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1985 Apr 11;13(7):2533–2544. doi: 10.1093/nar/13.7.2533

Monoclonal antibodies to DNA modified by 8-methoxypsoralen and ultraviolet A light.

R M Santella, N Dharmaraja, F P Gasparro, R L Edelson
PMCID: PMC341173  PMID: 4000963

Abstract

A panel of monoclonal antibodies have been developed which specifically recognize DNA modified by 8-methoxypsoralen (8-MOP) and ultraviolet A light (320-400 nm) (UVA). These antibodies have been characterized as to sensitivity and specificity by an enzyme linked immunosorbent assay (ELISA). In a competitive ELISA with the most sensitive antibody, 50% inhibition of antibody binding occurred at 17 fmole 8-MOP-DNA photo adducts. One adduct per 10(7) bases could be reliably detected. There was also some antibody cross-reactivity with DNAs modified by 4' aminomethyl-4, 5, 8-trimethylpsoralen and 4', 5-dimethylangelicin as well as DNA isolated from cells treated with 8-MOP and UVA. The primary specificity of one of the antibodies was shown to be the 4', 5' thymine monoadduct by competitive inhibition studies using HPLC fractions of an enzymatic digest of 8-MOP poly(dA-dT) . poly(dA-dT). These antibodies should allow the quantitation of adduct levels in various in vitro systems as well as humans exposed clinically to 8-MOP and UVA.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown H. S., Jeffrey A. M., Weinstein I. B. Formation of DNA adducts in 10T1/2 mouse embryo fibroblasts incubated with benzo(a)pyrene or dihydrodiol oxide derivatives. Cancer Res. 1979 May;39(5):1673–1677. [PubMed] [Google Scholar]
  2. Cleveland W. L., Wood I., Erlanger B. F. Routine large-scale production of monoclonal antibodies in a protein-free culture medium. J Immunol Methods. 1983 Jan 28;56(2):221–234. doi: 10.1016/0022-1759(83)90414-3. [DOI] [PubMed] [Google Scholar]
  3. Hanawalt P. C., Cooper P. K., Ganesan A. K., Smith C. A. DNA repair in bacteria and mammalian cells. Annu Rev Biochem. 1979;48:783–836. doi: 10.1146/annurev.bi.48.070179.004031. [DOI] [PubMed] [Google Scholar]
  4. Haugen A., Groopman J. D., Hsu I. C., Goodrich G. R., Wogan G. N., Harris C. C. Monoclonal antibody to aflatoxin B1-modified DNA detected by enzyme immunoassay. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4124–4127. doi: 10.1073/pnas.78.7.4124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hertzog P. J., Smith J. R., Garner R. C. Production of monoclonal antibodies to guanine imidazole ring-opened aflatoxin B1DNA, the persistent DNA adduct in vivo. Carcinogenesis. 1982;3(7):825–828. doi: 10.1093/carcin/3.7.825. [DOI] [PubMed] [Google Scholar]
  6. Leadon S. A., Hanawalt P. C. Monoclonal antibody to DNA containing thymine glycol. Mutat Res. 1983 Aug;112(4):191–200. doi: 10.1016/0167-8817(83)90006-8. [DOI] [PubMed] [Google Scholar]
  7. Parrish J. A., Fitzpatrick T. B., Tanenbaum L., Pathak M. A. Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light. N Engl J Med. 1974 Dec 5;291(23):1207–1211. doi: 10.1056/NEJM197412052912301. [DOI] [PubMed] [Google Scholar]
  8. Pearson T. W., Pinder M., Roelants G. E., Kar S. K., Lundin L. B., Mayor-Withey K. S., Hwett R. S. Methods for derivation and detection of anti-parasite monoclonal antibodies. J Immunol Methods. 1980;34(2):141–154. doi: 10.1016/0022-1759(80)90168-4. [DOI] [PubMed] [Google Scholar]
  9. Santella R. M., Lin C. D., Cleveland W. L., Weinstein I. B. Monoclonal antibodies to DNA modified by a benzo[a]pyrene diol epoxide. Carcinogenesis. 1984 Mar;5(3):373–377. doi: 10.1093/carcin/5.3.373. [DOI] [PubMed] [Google Scholar]
  10. Shindo H., Simpson R. T., Cohen J. S. An alternating conformation characterizes the phosphodiester backbone of poly(dA-dT) in solution. J Biol Chem. 1979 Sep 10;254(17):8125–8128. [PubMed] [Google Scholar]
  11. Sinden R. R., Cole R. S. Repair of cross-linked DNA and survival of Escherichia coli treated with psoralen and light: effects of mutations influencing genetic recombination and DNA metabolism. J Bacteriol. 1978 Nov;136(2):538–547. doi: 10.1128/jb.136.2.538-547.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Song P. S., Tapley K. J., Jr Photochemistry and photobiology of psoralens. Photochem Photobiol. 1979 Jun;29(6):1177–1197. doi: 10.1111/j.1751-1097.1979.tb07838.x. [DOI] [PubMed] [Google Scholar]
  13. Stern R. S., Laird N., Melski J., Parrish J. A., Fitzpatrick T. B., Bleich H. L. Cutaneous squamous-cell carcinoma in patients treated with PUVA. N Engl J Med. 1984 May 3;310(18):1156–1161. doi: 10.1056/NEJM198405033101805. [DOI] [PubMed] [Google Scholar]
  14. Strickland P. T., Boyle J. M. Characterisation of two monoclonal antibodies specific for dimerised and non-dimerised adjacent thymidines in single stranded DNA. Photochem Photobiol. 1981 Nov;34(5):595–601. [PubMed] [Google Scholar]
  15. Zarebska Z., Jarzabek-Chorzelska M., Rzesa G., Gliński W., Pawińska M., Chorzelski T., Jablońska S. Detection of DNA-psoralen photoadducts in situ. Photochem Photobiol. 1984 Mar;39(3):307–312. doi: 10.1111/j.1751-1097.1984.tb08182.x. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES