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. 1985 May 10;13(9):3335–3342. doi: 10.1093/nar/13.9.3335

Junction sequences generated by 'one-ended transposition'.

S Mötsch, R Schmitt, P Avila, F de la Cruz, E Ward, J Grinsted
PMCID: PMC341238  PMID: 2987883

Abstract

In the presence of the cognate transposase, plasmids containing a single inverted repeat (IR) sequence of Tn21 or of Tn1721 can fuse efficiently with other plasmids ('one-ended transposition'). The junctions across the sequences of donor and recipient DNA in recombinants generated by this process have been determined. These show that the segment of donor DNA starts precisely at the IR sequence (it is variable at the other end), and is flanked by a direct repeat of host DNA (usually 5bp) that was present only once in the original host sequence. These are characteristics of recombinants generated by transposition of Tn21 and Tn1721 themselves, suggesting that the mechanism of one-ended transposition is very similar to that of the corresponding entire elements.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arthur A., Nimmo E., Hettle S., Sherratt D. Transposition and transposition immunity of transposon Tn3 derivatives having different ends. EMBO J. 1984 Aug;3(8):1723–1729. doi: 10.1002/j.1460-2075.1984.tb02038.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arthur A., Sherratt D. Dissection of the transposition process: a transposon-encoded site-specific recombination system. Mol Gen Genet. 1979 Oct 1;175(3):267–274. doi: 10.1007/BF00397226. [DOI] [PubMed] [Google Scholar]
  3. Avila P., de la Cruz F., Ward E., Grinsted J. Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase. Mol Gen Genet. 1984;195(1-2):288–293. doi: 10.1007/BF00332761. [DOI] [PubMed] [Google Scholar]
  4. Galas D. J., Chandler M. On the molecular mechanisms of transposition. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4858–4862. doi: 10.1073/pnas.78.8.4858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harshey R. M., Bukhari A. I. A mechanism of DNA transposition. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1090–1094. doi: 10.1073/pnas.78.2.1090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mötsch S., Schmitt R. Replicon fusion mediated by a single-ended derivative of transposon Tn1721. Mol Gen Genet. 1984;195(1-2):281–287. doi: 10.1007/BF00332760. [DOI] [PubMed] [Google Scholar]
  7. Schöffl F., Arnold W., Pühler A., Altenbuchner J., Schmitt R. The tetracycline resistance transposons Tn1721 and Tn1771 have three 38-base-pair repeats and generate five-base-pair direct repeats. Mol Gen Genet. 1981;181(1):87–94. doi: 10.1007/BF00339010. [DOI] [PubMed] [Google Scholar]
  8. Swift G., McCarthy B. J., Heffron F. DNA sequence of a plasmid-encoded dihydrofolate reductase. Mol Gen Genet. 1981;181(4):441–447. doi: 10.1007/BF00428733. [DOI] [PubMed] [Google Scholar]
  9. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  10. Zheng Z. X., Chandler M., Hipskind R., Clerget M., Caro L. Dissection of the r-determinant of the plasmid R100.1: the sequence at the extremities of Tn21. Nucleic Acids Res. 1981 Dec 11;9(23):6265–6278. doi: 10.1093/nar/9.23.6265. [DOI] [PMC free article] [PubMed] [Google Scholar]

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