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. 1985 May 24;13(10):3651–3666. doi: 10.1093/nar/13.10.3651

Improved rapid phosphotriester synthesis of oligodeoxyribonucleotides using oxygen-nucleophilic catalysts.

V A Efimov, O G Chakhmakhcheva, Ovchinnikov YuA
PMCID: PMC341264  PMID: 4011438

Abstract

The use of different condensing and phosphorylating agents in conjunction with oxygen-nucleophilic catalysts, such as 4-substituted derivatives of pyridine N-oxide and quinoline N-oxide, leads to a dramatic increase of the rate of the phosphotriester bond formation and minimizes the amount of by-products caused by the modification of heterocyclic bases. The application of these catalysts to the solid-phase oligonucleotide synthesis allows to reduce the time needed for the performance of one elongation cycle on a polymer support to 10 min.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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