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. 2012 Jul 2;1(7):e19. doi: 10.1038/oncsis.2012.19

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Copyright © 2012 Macmillan Publishers Limited

This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Efficient expression of NR4A1 and NR4A3 in human AML cells using direct IVT-RNA electroporation. (a) Flow cytometry analysis reveals high transfection efficiency (93%) of IVT-EGFP into Kasumi-1 cells with minimal increase in cell death 24 h after electroporation as compared with no electroporation control. (b) Fluorescent microscopy images of IVT-EGFP electroporated Kasumi-1 cells. (c) Time-course western blot analysis of Kasumi-1 cells electroporated with EGFP and Luciferase, NR4A1 or NR4A3 IVT-RNAs.