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. 1985 Jun 11;13(11):3823–3839. doi: 10.1093/nar/13.11.3823

Overproduction of the EcoR V endonuclease and methylase.

L Bougueleret, M L Tenchini, J Botterman, M Zabeau
PMCID: PMC341280  PMID: 2989776

Abstract

Strains overproducing the EcoR V endonuclease and methylase have been obtained by inserting each of the two genes in expression vectors containing the lambda PL promoter. The methylase is overproduced to a level reaching 5-10% of the total cellular proteins, which represents a 50-100 fold increase. A 30 fold overproduction of endonuclease was achieved by randomly positioning the EndRV gene downstream of the lambda PL promoter. The situation in the endonuclease overproducing clone resembles that encountered in maxi-cells. The strains described here allowed a quick purification of both enzymes in sufficient amounts for crystallisation attempts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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