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. 1985 Jun 11;13(11):3861–3871. doi: 10.1093/nar/13.11.3861

A new copia-like transposable element found in a Drosophila rDNA gene unit.

J R Bell, A M Bogardus, T Schmidt, M Pellegrini
PMCID: PMC341282  PMID: 2989778

Abstract

We have discovered a member of a new family of copia-like transposable elements inserted into the non-transcribed spacer between two ribosomal genes (rDNA). This family, which we call 3S18, consists of at least 15 elements which are scattered throughout the Drosophila melanogaster genome. The elements of this family are approximately 6.5 kb long and have 0.5 kb terminal direct repeats. All of the elements appear to have the same restriction sites. The element is mobile as the size pattern of homologous fragments varies among different strains. In situ hybridization results confirm the scattered location and transposable qualities of 3S18. The element is not transcribed into abundant RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnes W. M. Plasmid detection and sizing in single colony lysates. Science. 1977 Jan 28;195(4276):393–394. doi: 10.1126/science.318764. [DOI] [PubMed] [Google Scholar]
  3. Blin N., Stafford D. W. A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 1976 Sep;3(9):2303–2308. doi: 10.1093/nar/3.9.2303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dagert M., Ehrlich S. D. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. doi: 10.1016/0378-1119(79)90082-9. [DOI] [PubMed] [Google Scholar]
  5. Fabijanski S., Pellegrini M. A Drosophila ribosomal protein gene is located near repeated sequences including rDNA sequences. Nucleic Acids Res. 1982 Oct 11;10(19):5979–5991. doi: 10.1093/nar/10.19.5979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fabijanski S., Pellegrini M. Isolation of a cloned DNA segment containing a ribosomal protein gene of Drosophila melanogaster. Gene. 1982 Jun;18(3):267–276. doi: 10.1016/0378-1119(82)90165-2. [DOI] [PubMed] [Google Scholar]
  7. Kidd S. J., Glover D. M. Drosophila melanogaster ribosomal DNA containing type II insertions is variably transcribed in different strains and tissues. J Mol Biol. 1981 Oct 5;151(4):645–662. doi: 10.1016/0022-2836(81)90428-9. [DOI] [PubMed] [Google Scholar]
  8. Kidd S., Lockett T. J., Young M. W. The Notch locus of Drosophila melanogaster. Cell. 1983 Sep;34(2):421–433. doi: 10.1016/0092-8674(83)90376-8. [DOI] [PubMed] [Google Scholar]
  9. Levis R., Collins M., Rubin G. M. FB elements are the common basis for the instability of the wDZL and wC Drosophila mutations. Cell. 1982 Sep;30(2):551–565. doi: 10.1016/0092-8674(82)90252-5. [DOI] [PubMed] [Google Scholar]
  10. Long E. O., Dawid I. B. Expression of ribosomal DNA insertions in Drosophila melanogaster. Cell. 1979 Dec;18(4):1185–1196. doi: 10.1016/0092-8674(79)90231-9. [DOI] [PubMed] [Google Scholar]
  11. Long E. O., Dawid I. B. Repeated genes in eukaryotes. Annu Rev Biochem. 1980;49:727–764. doi: 10.1146/annurev.bi.49.070180.003455. [DOI] [PubMed] [Google Scholar]
  12. Long E. O., Dawid I. B. Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster. Nucleic Acids Res. 1979 Sep 11;7(1):205–215. doi: 10.1093/nar/7.1.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mattox W. W., Davidson N. Isolation and characterization of the Beadex locus of Drosophila melanogaster: a putative cis-acting negative regulatory element for the heldup-a gene. Mol Cell Biol. 1984 Jul;4(7):1343–1353. doi: 10.1128/mcb.4.7.1343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. O'Hare K., Murphy C., Levis R., Rubin G. M. DNA sequence of the white locus of Drosophila melanogaster. J Mol Biol. 1984 Dec 15;180(3):437–455. doi: 10.1016/0022-2836(84)90021-4. [DOI] [PubMed] [Google Scholar]
  16. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  17. Rubin G. M., Brorein W. J., Jr, Dunsmuir P., Flavell A. J., Levis R., Strobel E., Toole J. J., Young E. Copia-like transposable elements in the Drosophila genome. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):619–628. doi: 10.1101/sqb.1981.045.01.080. [DOI] [PubMed] [Google Scholar]
  18. Santon J. B., Pellegrini M. Rates of ribosomal protein and total protein synthesis during Drosophila early embryogenesis. Dev Biol. 1981 Jul 15;85(1):252–257. doi: 10.1016/0012-1606(81)90255-4. [DOI] [PubMed] [Google Scholar]
  19. Sharp P. A., Hsu M. T., Otsubo E., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol. 1972 Nov 14;71(2):471–497. doi: 10.1016/0022-2836(72)90363-4. [DOI] [PubMed] [Google Scholar]
  20. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  21. Spradling A., Pardue M. L., Penman S. Messenger RNA in heat-shocked Drosophila cells. J Mol Biol. 1977 Feb 5;109(4):559–587. doi: 10.1016/s0022-2836(77)80091-0. [DOI] [PubMed] [Google Scholar]
  22. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wong Y. C., O'Connell P., Rosbash M., Elgin S. C. DNase I hypersensitive sites of the chromatin for Drosophila melanogaster ribosomal protein 49 gene. Nucleic Acids Res. 1981 Dec 21;9(24):6749–6762. doi: 10.1093/nar/9.24.6749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zachar Z., Bingham P. M. Regulation of white locus expression: the structure of mutant alleles at the white locus of Drosophila melanogaster. Cell. 1982 Sep;30(2):529–541. doi: 10.1016/0092-8674(82)90250-1. [DOI] [PubMed] [Google Scholar]

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