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. Author manuscript; available in PMC: 2013 Sep 18.
Published in final edited form as: Neuroscience. 2012 Jun 19;220:47–61. doi: 10.1016/j.neuroscience.2012.06.026

FIGURE 5. The glycosylation of RPTPζ/phosphacan is seemingly normal in Large myodysotrophy mutant mice.

FIGURE 5

Whole brains from P1 +/+ and Largemyd mutant mice (−/−) that were either +/+ or +/− for POMGnT1 were homogenized and processed for subcellular fractionation. The resulting soluble fraction was analyzed by Western blot analysis for Cat-315, 3F8, H300, 5210, HNK-1, and brevican. In all cases, large molecular weight bands positive for Cat-315, 3F8, H300, 5210 and HNK-1 resolved well above 250kD. An antibody detecting the full-length form of brevican was used as a loading control and reactive bands were detected around 150kD. Quantification of reactive bands revealed no alteration in their intensities when normalized to brevican for all antibodies with respect to genotype. Additionally, the molecular weight of H300 in Large myodystrophy mutant mice was not unaltered from controls samples.