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. 1986 Dec 22;14(24):9797–9809. doi: 10.1093/nar/14.24.9797

A bacterial protein requirement for the bacteriophage lambda terminase reaction.

M Gold, W Parris
PMCID: PMC341336  PMID: 3027664

Abstract

The bacteriophage lambda terminase enzyme cleaves the cohesive-end sites of lambda DNA to yield the protruding 5'-termini of the mature molecule. In vitro, this endonucleolytic event requires a protein factor which has been isolated and purified from extracts of uninfected E. coli. The terminase host factor (THF) is a heat stable basic protein of M.W. approximately 22,000. The integration host factor (IHF) protein of E. coli can efficiently substitute for THF in the terminase reaction; however, THF can be demonstrated to be physically present in, and isolated with full biological activity from extracts of cells defective or deficient in IHF.

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