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. 1986 Dec 22;14(24):9965–9976. doi: 10.1093/nar/14.24.9965

Studies towards the development of chemically synthesized non-radioactive biotinylated nucleic acid hybridization probes.

A H Al-Hakim, R Hull
PMCID: PMC341348  PMID: 3027670

Abstract

Non-radioactive nucleic acid hybridization probes have been constructed in which the reporter group is long chain biotin chemically linked to a basic macromolecule (histone H1, cytochrome C or polyethyleneimine). The modified basic macromolecule which carries many biotin residues can, in turn, be covalently linked to nucleic acids (DNA) via the bifunctional cross-linking reagents, glutaraldehyde, 1,2,7,8-diepoxyoctane, bis (succinimidyl) suberate or bis (sulfonosuccinimidyl) suberate. This provides a very sensitive probe by which as little as between 10-50fg of target DNA can be visualized using dot-blot hybridization procedures in conjunction with avidin or streptavidin enzyme conjugates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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