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. 1986 Dec 22;14(24):10001–10008. doi: 10.1093/nar/14.24.10001

Nucleotide sequence of Bacillus phage phi 29 genes 14 and 15: homology of gene 15 with other phage lysozymes.

K J Garvey, M S Saedi, J Ito
PMCID: PMC341351  PMID: 3027653

Abstract

The nucleotide sequence of Bacillus phage phi 29 genes 14 (g14) and 15 (g15) have been determined and shown to encode proteins with molecular weights of 15,014 and 28,022, respectively. The g14 open reading frame (ORF) was confirmed by sequencing a sus14(1241) mutant. Gene product 15 (gp15) has considerable homology with Salmonella phage P22 lysozyme and lesser homology with Escherichia coli phage T4 lysozyme. Putative translation signals are identified. In addition, the role of a previously described promoter, B2, is discussed.

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Selected References

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  1. Bieńkowska-Szewczyk K., Taylor A. Murein transglycosylase from phage lambda lysate. Purification and properties. Biochim Biophys Acta. 1980 Oct;615(2):489–496. doi: 10.1016/0005-2744(80)90515-x. [DOI] [PubMed] [Google Scholar]
  2. Bjornsti M. A., Reilly B. E., Anderson D. L. In vitro assembly of the Bacillus subtilis bacteriophage phi 29. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5861–5865. doi: 10.1073/pnas.78.9.5861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bjornsti M. A., Reilly B. E., Anderson D. L. Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: prohead restoration for DNA-gp3 packaging and assembly. J Virol. 1985 Mar;53(3):858–861. doi: 10.1128/jvi.53.3.858-861.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Camacho A., Jiménez F., De La Torre J., Carrascosa J. L., Mellado R. P., Vásquez C., Viñuela E., Salas M. Assembly of Bacillus subtilis phage phi29. 1. Mutants in the cistrons coding for the structural proteins. Eur J Biochem. 1977 Feb 15;73(1):39–55. doi: 10.1111/j.1432-1033.1977.tb11290.x. [DOI] [PubMed] [Google Scholar]
  5. Carrascosa J. L., Camacho A., Moreno F., Jiménez F., Mellado R. P., Viñuela E., Salas M. Bacillus subtilis phage phi29. Characterization of gene products and functions. Eur J Biochem. 1976 Jul 1;66(2):229–241. doi: 10.1111/j.1432-1033.1976.tb10512.x. [DOI] [PubMed] [Google Scholar]
  6. Coleman J., Green P. J., Inouye M. The use of RNAs complementary to specific mRNAs to regulate the expression of individual bacterial genes. Cell. 1984 Jun;37(2):429–436. doi: 10.1016/0092-8674(84)90373-8. [DOI] [PubMed] [Google Scholar]
  7. Escarmís C., Salas M. Nucleotide sequence of the early genes 3 and 4 of bacteriophage phi 29. Nucleic Acids Res. 1982 Oct 11;10(19):5785–5798. doi: 10.1093/nar/10.19.5785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Garvey K. J., Saedi M. S., Ito J. The complete sequence of Bacillus phage phi 29 gene 16: a protein required for the genome encapsidation reaction. Gene. 1985;40(2-3):311–316. doi: 10.1016/0378-1119(85)90054-x. [DOI] [PubMed] [Google Scholar]
  9. Garvey K. J., Yoshikawa H., Ito J. The complete sequence of the Bacillus phage phi 29 right early region. Gene. 1985;40(2-3):301–309. doi: 10.1016/0378-1119(85)90053-8. [DOI] [PubMed] [Google Scholar]
  10. Hawley L. A., Reilly B. E., Hagen E. W., Anderson D. L. Viral protein synthesis in bacteriophage phi 29-infected Bacillus subtilis. J Virol. 1973 Nov;12(5):1149–1159. doi: 10.1128/jvi.12.5.1149-1159.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Inouye M., Tsugita A. Amino acid sequence of T2 phage lysozyme. J Mol Biol. 1968 Oct 14;37(1):213–223. doi: 10.1016/0022-2836(68)90084-3. [DOI] [PubMed] [Google Scholar]
  12. Jiménez F., Camacho A., De La Torre J., Viñuela E., Salas M. Assembly of Bacillus subtilis phage phe29. 2. Mutants in the cistrons coding for the non-structural proteins. Eur J Biochem. 1977 Feb 15;73(1):57–72. doi: 10.1111/j.1432-1033.1977.tb11291.x. [DOI] [PubMed] [Google Scholar]
  13. Josslin R. The lysis mechanism of phage T4: mutants affecting lysis. Virology. 1970 Mar;40(3):719–726. doi: 10.1016/0042-6822(70)90216-3. [DOI] [PubMed] [Google Scholar]
  14. Kawamura F., Ito J. Transcription of the genome of bacteriophage phi 29: isolation and mapping of the major early mRNA synthesized in vivo and in vitro. J Virol. 1977 Sep;23(3):562–577. doi: 10.1128/jvi.23.3.562-577.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lipman D. J., Pearson W. R. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. doi: 10.1126/science.2983426. [DOI] [PubMed] [Google Scholar]
  16. Matthews B. W., Remington S. J., Grütter M. G., Anderson W. F. Relation between hen egg white lysozyme and bacteriophage T4 lysozyme: evolutionary implications. J Mol Biol. 1981 Apr 25;147(4):545–558. doi: 10.1016/0022-2836(81)90399-5. [DOI] [PubMed] [Google Scholar]
  17. Mellado R. P., Barthelemy I., Salas M. In vivo transcription of bacteriophage phi 29 DNA early and late promoter sequences. J Mol Biol. 1986 Sep 20;191(2):191–197. doi: 10.1016/0022-2836(86)90256-1. [DOI] [PubMed] [Google Scholar]
  18. Mellado R. P., Moreno F., Viñuela E., Salas M., Reilly B. E., Anderson D. L. Genetic analysis of bacteriophage phi 29 of Bacillus subtilis: integration and mapping of reference mutants of two collections. J Virol. 1976 Aug;19(2):495–500. doi: 10.1128/jvi.19.2.495-500.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moran C. P., Jr, Lang N., LeGrice S. F., Lee G., Stephens M., Sonenshein A. L., Pero J., Losick R. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol Gen Genet. 1982;186(3):339–346. doi: 10.1007/BF00729452. [DOI] [PubMed] [Google Scholar]
  20. Mount D. W., Conrad B. Microcomputer programs for graphic analysis of nucleic acid and protein sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):811–817. doi: 10.1093/nar/12.1part2.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Owen J. E., Schultz D. W., Taylor A., Smith G. R. Nucleotide sequence of the lysozyme gene of bacteriophage T4. Analysis of mutations involving repeated sequences. J Mol Biol. 1983 Apr 5;165(2):229–248. doi: 10.1016/s0022-2836(83)80255-1. [DOI] [PubMed] [Google Scholar]
  22. Paces V., Hostomský Z., Fucík V., Pivec L., Zadrazil S. Comparison of Bacillus subtilis phages PZA, phi 29 and phi 15. Folia Biol (Praha) 1984;30(Spec No):52–64. [PubMed] [Google Scholar]
  23. Rao G. R., Burma D. P. Purification and properties of phage P22-induced lysozyme. J Biol Chem. 1971 Nov;246(21):6474–6479. [PubMed] [Google Scholar]
  24. Reader R. W., Siminovitch L. Lysis defective mutants of bacteriophage lambda: genetics and physiology of S cistron mutants. Virology. 1971 Mar;43(3):607–622. doi: 10.1016/0042-6822(71)90286-8. [DOI] [PubMed] [Google Scholar]
  25. Reilly B. E., Nelson R. A., Anderson D. L. Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: mapping and functional analysis of the head fiber gene. J Virol. 1977 Oct;24(1):363–377. doi: 10.1128/jvi.24.1.363-377.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rennell D., Poteete A. R. Phage P22 lysis genes: nucleotide sequences and functional relationships with T4 and lambda genes. Virology. 1985 May;143(1):280–289. doi: 10.1016/0042-6822(85)90115-1. [DOI] [PubMed] [Google Scholar]
  27. Simons R. W., Kleckner N. Translational control of IS10 transposition. Cell. 1983 Sep;34(2):683–691. doi: 10.1016/0092-8674(83)90401-4. [DOI] [PubMed] [Google Scholar]
  28. Smith D. R., Calvo J. M. Nucleotide sequence of the E coli gene coding for dihydrofolate reductase. Nucleic Acids Res. 1980 May 24;8(10):2255–2274. doi: 10.1093/nar/8.10.2255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sogo J. M., Inciarte M. R., Corral J., Viñuela E., Salas M. RNA polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage phi29. J Mol Biol. 1979 Feb 5;127(4):411–436. doi: 10.1016/0022-2836(79)90230-4. [DOI] [PubMed] [Google Scholar]
  30. Tinoco I., Jr, Borer P. N., Dengler B., Levin M. D., Uhlenbeck O. C., Crothers D. M., Bralla J. Improved estimation of secondary structure in ribonucleic acids. Nat New Biol. 1973 Nov 14;246(150):40–41. doi: 10.1038/newbio246040a0. [DOI] [PubMed] [Google Scholar]
  31. Tomizawa J. Control of ColE1 plasmid replication: the process of binding of RNA I to the primer transcript. Cell. 1984 Oct;38(3):861–870. doi: 10.1016/0092-8674(84)90281-2. [DOI] [PubMed] [Google Scholar]
  32. Tsugita A., Inouye M. Purification of bacteriophage T4 lysozyme. J Biol Chem. 1968 Jan 25;243(2):391–397. [PubMed] [Google Scholar]
  33. Weaver L. H., Rennell D., Poteete A. R., Mathews B. W. Structure of phage P22 gene 19 lysozyme inferred from its homology with phage T4 lysozyme. Implications for lysozyme evolution. J Mol Biol. 1985 Aug 20;184(4):739–741. doi: 10.1016/0022-2836(85)90318-3. [DOI] [PubMed] [Google Scholar]

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