Figure 7 .

Effect of divalent metal ion on the vanadate-mediated V1 photocleavage pattern of wild-type and mutant dynein. (A) Representative silver-stained gel images showing vanadate photocleavage of DHC from wild-type (left panels) and AAA3 E/Q (right panels) under V1 Mg²⁺ and V1 Mn²⁺ conditions. High- and low-molecular-weight UV cleavage products are indicated by “_*” and “>”, respectively. Additional prominent cleavage products found under AAA3 E/Q V1 Mn²⁺ conditions are indicated by “►”. (B) Quantification of the percentage of dynein cleaved in comparison to the total dynein in the sample. Percentage cleavage was quantified from three independent experiments. Data are shown as mean ± SEM.