Whole-Genome Sequence of Staphylococcus aureus Strain LCT-SA112

Abstract

Staphylococcus aureus is a facultative anaerobic Gram-positive coccal bacterium. S. aureus is the most common species of Staphylococcus to cause staphylococcal infections, which are very common in clinical medicine. Here we report the genome sequence of S. aureus strain LCT-SA112, which was isolated from S. aureus subsp. aureus CGMCC 1.230.

GENOME ANNOUNCEMENT

The genus Staphylococcus contains facultative Gram-positive and ball-shaped bacteria that are commonly found in soil, air, and water. The species of the genus Staphylococcus are important opportunistic pathogens of both animals and humans (7). Particularly, methicillin resistance has emerged as an important issue, with significant concerns about public health (6). Staphylococcus aureus subsp. aureus CGMCC 1.2386 was originally obtained from the China General Microbiological Culture Collection. However, subsequent cultivation showed that this strain was contaminated with other bacteria because we noticed two kinds of colonies on a medium plate. After purification and identification by16S rRNA gene sequencing, we identified one of the colonies as S. aureus and the strain was designated LCT-SA112.

Whole-genome shotgun (WGS) sequencing of S. aureus DNA was performed by BGI-Shenzhen (Shenzhen, People's Republic of China) using Solexa paired-end sequencing technology (1, 8). Ninety base pairs were set for the 350-bp PCR-free index library and the 6,000-bp index library. WGS sequence data of 315 Mb, giving approximately 110-fold genome coverage, were generated and assembled into 72 contigs by using SOAPdenovo v1.05 (4). Furthermore, the contigs were joined into six scaffolds (>500 bp in size) using paired-end information.

The unclosed draft genome sequence analysis of S. aureus showed a genome size of 2,793,131 bp with a mean GC content of 32.70%, and the published genome size of S. aureus is about 2.8 Mb (3). Annotation of the open reading frames was performed by using Glimmer v.3.0 (2) and by comparison with the corresponding data from the COG, KEGG, GO, Swiss-Prot, TrEMBL, and NCBI nr databases. We found 2,607 coding sequences (CDSs) with a GC content of 33.57%. There were 2,015 CDSs involving the 21 functional COG groups and a part of the CDSs involving the 29 metabolic pathway KEGG groups. Meanwhile, we have done Gene GO annotation of 1,679 CDSs and found that 19 functional categories enrich the LCT-SA112 sample. In addition, 37 tRNAs were identified by tRNAscan-SE (5); 10 sRNAs were also found. Furthermore, 134 tandem repeats were predicted.

Nucleotide sequence accession numbers.

This WGS project has been deposited at DDBJ/EMBL/GenBank under accession no. AJLP00000000. The version described in this paper is the first version, AJLP01000000.