Abstract
The psychrotolerant Pseudoalteromonas issachenkonii PAMC 22718 was isolated for its higher chitinase and protease activities from cold seawater in the Kara Sea, Arctic. Here, we present the draft genome sequence of PAMC 22718 to provide further information for the ecological function of the genus Pseudoalteromonas in a cold marine environment.
GENOME ANNOUNCEMENT
Chitin and proteins and their constituent amino acids are the most abundant natural substances in cold marine systems and serve as nutrient sources for marine microbes (3, 5). Thus, many cold-adapted chitinolytic and proteolytic bacteria have been isolated from various marine habitats (2, 6). Marine heterotrophic bacteria of the genus Pseudoalteromonas, comprising one of the most abundant groups of Proteobacteria, are widely distributed in the marine environment. Current evidence suggests that psychrophilic and/or psychrotolerant Pseudoalteromonas bacteria are believed to play a critical role in the carbon and nitrogen recycling process due to their cold-active enzymes.
The genome of Pseudoalteromonas issachenkonii PAMC 22718 was analyzed using a 150-bp paired-end library (5,495,145 reads) with the Illumina Genome Analyzer II× (Illumina, San Diego, CA). The genome assembly was performed using Celera assembler 7.0 (4). Gene prediction and annotation were performed using the RAST server (1). The draft genome sequence of P. issachenkonii PAMC 22718 was approximately 4.2 Mb long and comprised 56 contigs with an average G+C content of 39.91%. The resulting N50 size of contigs was 127,821 bp and the total coverage over the genome was 131-fold. A total of 3,944 open reading frames (ORFs), 76 tRNA genes, and 7 rRNA genes were predicted in the draft genome. Approximately 84.84% of nucleotides were predicted as ORFs, and 3,182 (79.67%) ORFs were annotated with known proteins.
Nucleotide sequence accession numbers.
This Whole Genome Shotgun project has been deposited in DDBJ/EMBL/GenBank under the accession number AJTK00000000. The version described in this paper is the first version, AJTK01000000.
ACKNOWLEDGMENTS
This work was supported by the grants to the Korea Polar Research Institute under projects PM11090 and PE11190.
REFERENCES
- 1. Aziz RK, et al. 2008. The RAST server: rapid annotations using subsystems technology. BMC Genomics 9:75. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2. Chen XL, Xie BB, Lu JT, He HL, Zhang Y. 2007. A novel type of subtilase from the psychrotolerant bacterium Pseudoalteromonas sp. SM9913: catalytic and structural properties of deseasin MCP-01. Microbiology 153:2116–2125 [DOI] [PubMed] [Google Scholar]
- 3. Kurita K. 2001. Controlled functionalization of the polysaccharide chitin. Prog. Polym. Sci. 26:1921–1971 [Google Scholar]
- 4. Myers EW, et al. 2000. A whole-genome assembly of Drosophila. Science 287:2196. [DOI] [PubMed] [Google Scholar]
- 5. Nguyen RT, Harvey HR. 1997. Protein and amino acid cycling during phytoplankton decomposition in oxic and anoxic waters. Org. Geochem. 27:115–128 [Google Scholar]
- 6. Stefanidi E, Vorgias CE. 2008. Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles 12:541–552 [DOI] [PubMed] [Google Scholar]