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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Virology. 2012 May 8;429(2):155–162. doi: 10.1016/j.virol.2012.04.008

Fig. 5.

Fig. 5

Effect of Tetherin on HIV-1 VLP release is dependent on Gag expression level. 293T cells were transfected either with one of two fixed amounts (200ng (A and B) and 25ng (C and D) of a plasmid expressing Gag-α along with increasing amounts (0 to 200ng) of a tetherin expression plasmid. Cells and VLPs were subjected to β-galactosidase assay (A and C) and western blot analysis (B and D) with anti-Gag, anti-Tubulin and anti-HA antibodies. The graph in (E) shows the fold reduction in Gag-α VLP yield (as measured by β-galactosidase assay) attributable to tetherin for each amount of Gag-α expression plasmid (200ng and 25ng).