FIG 2 .
Producing EPS (exopolysaccharide) as a shunt pathway for toxic metabolites from galactose. (A to C) Characterization of galE suppressor mutants resistant to galactose toxicity. Cells were inoculated on LB agar plates supplemented with 0.5% galactose. (D) Overexpression of the epsA-O operon suppressed galactose toxicity in the galE mutant. galE::tet Physpank-epsA-O cells (YC776) were grown in MSgg media in the absence (left) or presence (right) of 0.1% galactose and with various amounts of IPTG (see the key). The galE epsA-O double mutant is also included as a control. (E) Sugar composition analysis of EPS from wild-type (3610) or ΔepsA-O mutant (YC771) pellicles. Glc, glucose; GalNAc, N-acetyl-galactose; Gal, galactose.