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. 1978 Jan;5(1):1–11. doi: 10.1093/nar/5.1.1

Studies of the transcription of viral genome in adenovirus 5 transformed cells.

E I Frolova, E S Zalmanzon, E M Lukanidin, G P Georgiev
PMCID: PMC341957  PMID: 643600

Abstract

Transcription of the human adenovirus 5 genome in transformed rat embryo cells (DFK3) was investigated using two different approaches. Preferential digestion of transcribed viral sequences by DNase I was analysed using kinetics of renaturation of 32P-labeled Ad5 HpaI restriction fragments in the presence of material which was stable after nuclease treatment. The second approach was the hybridization of 32P-labeled nuclear RNA from transformed cells with Ad5 restriction fragments which were attached to a nitrocellulose filter. These two methods gave similar results. It was found that not all integrated regions of the Ad5 genome are active in transformed cells. 2,5 copies of the HpaI-E fragment of Ad5 DNA were found in transformed DFK3 cell line. Nuclear RNA from these cells hybridized to HpaI-E fragment of Ad5 DNA, but only about half of sequences of the integrated HpaI-E fragment was sensitive to DNase I digestion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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