Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1978 Jan;5(1):195–204. doi: 10.1093/nar/5.1.195

Two initiation sites for adenovirus 5.5S RNA.

B Vennström, U Pettersson, L Philipson
PMCID: PMC341971  PMID: 643608

Abstract

Adenovirus specific 5.5S RNA is heterogeneous at its 5' terminus. Complete pancreatic RNase digests of the RNA reveal a 5' terminal oligonucleotide (pp)pApGpCp in addition to the major 5' terminal (pp)pGpGpGpCp (1). Both 5' termini are detected early as well as late after adenovirus infection. In isolated nuclei, alpha-amanitin inhibits all 5.5S RNA transcription at a concentration of 200 microgram/ml, indicating that both initiation sites are recognized by RNA polymerase III.

Full text

PDF
195

Images in this article

197Image
on p.197
198Image
on p.198
199Image
on p.199
199Image
on p.199
201Image
on p.201
201Image
on p.201
202Image
on p.202

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Franklin R. M. Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504–1511. doi: 10.1073/pnas.55.6.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hatlen L. E., Amaldi F., Attardi G. Oligonucleotide pattern after pancreatic ribonuclease digestion and the 3' and 5' termini of 5S ribonucleic acid from HeLa cells. Biochemistry. 1969 Dec;8(12):4989–5005. doi: 10.1021/bi00840a048. [DOI] [PubMed] [Google Scholar]
  3. Holmes D. S., Bonner J. Preparation, molecular weight, base composition, and secondary structure of giant nuclear ribonucleic acid. Biochemistry. 1973 Jun 5;12(12):2330–2338. doi: 10.1021/bi00736a023. [DOI] [PubMed] [Google Scholar]
  4. Kramer R. A., Steitz J. A. Correction for non-uniform phosphate labeling of E. coli and phage RNAs synthesized in vivo. Biochem Biophys Res Commun. 1973 Oct 1;54(3):1198–1202. doi: 10.1016/0006-291x(73)90819-x. [DOI] [PubMed] [Google Scholar]
  5. Lindberg U., Persson T., Philipson L. Isolation and characterization of adenovirus messenger ribonucleic acid in productive infection. J Virol. 1972 Nov;10(5):909–919. doi: 10.1128/jvi.10.5.909-919.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  7. Mathews M. B. Genes for VA-RNA in adenovirus 2. Cell. 1975 Oct;6(2):223–229. doi: 10.1016/0092-8674(75)90013-6. [DOI] [PubMed] [Google Scholar]
  8. Ohe K., Weissman S. M. The nucleotide sequence of a low molecular weight ribonucleic acid from cells infected with adenovirus 2. J Biol Chem. 1971 Nov 25;246(22):6991–7009. [PubMed] [Google Scholar]
  9. Pettersson U., Philipson L. Location of sequences on the adenovirus genome coding for the 5.5S RNA. Cell. 1975 Sep;6(1):1–4. doi: 10.1016/0092-8674(75)90066-5. [DOI] [PubMed] [Google Scholar]
  10. Pettersson U., Philipson L. Synthesis of complementary RNA sequences during productive adenovirus infection. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4887–4891. doi: 10.1073/pnas.71.12.4887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Price R., Penman S. A distinct RNA polymerase activity, synthesizing 5-5 s, 5 s and 4 s RNA in nuclei from adenovirus 2-infected HeLa cells. J Mol Biol. 1972 Oct 14;70(3):435–450. doi: 10.1016/0022-2836(72)90551-7. [DOI] [PubMed] [Google Scholar]
  12. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  13. Söderlund H., Pettersson U., Vennström B., Philipson L., Mathews M. B. A new species of virus-coded low molecular weight RNA from cells infected with adenovirus type 2. Cell. 1976 Apr;7(4):585–593. doi: 10.1016/0092-8674(76)90209-9. [DOI] [PubMed] [Google Scholar]
  14. Varricchio F., Sehulster L. M., Raska K., Jr Studies on synthesis and structure of virus-coded low molecular weight RNA in adenovirus type 2-infected KB cells. Virology. 1976 Oct 15;74(2):386–393. doi: 10.1016/0042-6822(76)90344-5. [DOI] [PubMed] [Google Scholar]
  15. Vennström B., Philipson L. Fidelity of adenovirus RNA transcription in isolated HeLa cell nuclei. J Virol. 1977 May;22(2):290–299. doi: 10.1128/jvi.22.2.290-299.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Volckaert G., Jou W. M., Fiers W. Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure. Anal Biochem. 1976 May 7;72:433–446. doi: 10.1016/0003-2697(76)90551-0. [DOI] [PubMed] [Google Scholar]
  17. Weinmann R., Brendler T. G., Raskas H. J., Roeder R. G. Low molecular weight viral RNAs transcribed by RNA polymerase III during adenovirus 2 infection. Cell. 1976 Apr;7(4):557–566. doi: 10.1016/0092-8674(76)90206-3. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES