Correspondence to: jv282@cscr.cam.ac.uk
Correction to: The EMBO Journal (2012) 31, 3431–3441. doi: 10.1038/emboj.2012.192
We noticed that the AOP version of this article contained an incomplete list of antibodies. Thus, the ‘Antibodies' and ‘Flow cytometry' paragraphs of the Materials and methods section have been replaced with complete details and explanations.
The authors apologise for any inconvenience caused.
Antibodies
For western blotting and immunohistochemistry. Anti-human CC-10 (Santa Cruz, SC-25554), anti-human SPC (Santa Cruz, SC-7705), anti-human AQP-5 (Santa Cruz, SC-28628), anti-human LGR6 (Santa Cruz, SC-48236), anti-human LGR5 (Santa Cruz, SC-68580), anti-human LGR5 (Sigma, HPA012530), anti-human Integrinα6 (Santa Cruz, SC-10730), anti-human C-Kit (Dako, A4502), anti-human Sox-9 (Millipore, AB5535), anti-human mitochondrial Ab (Thermo, MAS-12017), mouse anti-human nuclei Ab (Millipore, MAB1281), TTF-1 (Abcam, ab 40880), α-tubulin (Sigma, T9026), p38α (Cell Signaling, 9228), MAPKAPK2 (Cell Signaling, 3042), P-MAPKAPK2 (Cell Signaling, 3007), p-53 (Cell Signaling, 2524), p-p53 (Cell Signaling, 9287), C/EBPα (Santa Cruz, sc-61), Integrin β-1 (Santa Cruz, sc-6622).
For flow cytometry and immunofluorescence. Single-cell FACS was performed (MoFlo, Dako) following incubation with anti-human LGR6 (Santa Cruz, SC-99123), anti-E-cadherin (CD324, Biolegend, 50-3249), anti-Integrinα6 (CD49f, Biolegend; 12-0495), anti-αSMA (Sigma, F3777), anti-CD31 (Biolegend, 303104), anti-CD34 (Biolegend, 316410), anti-C-Kit (CD117, Biolegend, Cat: 323416), anti-C-Kit (CD117, Biolegend, Cat: 313202), anti-CD73 (Abcam, ab54217), anti-CD45 (Biolegend, 304006), anti-human CD105 (Biolegend, 323204), anti-CD117 (Biolegend, 313202), and anti-human e-cadherin (AbCam, Ab53033) antibodies.
Secondary antibodies. All the corresponding secondary antibodies (mouse, rabbit, goat, rat, 488, 555, 594, 647) were Alexa Fluor from Invitrogen and PE from Santa Cruz.