Abstract
Purpose
To determine the retinal and subretinal features characteristic to pseudoxanthoma elasticum (PXE) compared with age-related macular degeneration by using spectral-domain optical coherence tomography (SD-OCT) in Japanese patients.
Methods
We reviewed colour fundus photographs, fluorescein angiograms, and SD-OCT images of 52 eyes (27 Japanese patients) with angioid streaks (AS) due to PXE. Then we compared the incidence of tomographic features between 24 eyes (24 patient) with choroidal neovascularization (CNV) secondary to AS and 44 eyes (44 patients) with CNV secondary to age-related macular degeneration (AMD).
Results
Secondary CNV was found in 44 eyes (84.6%) of 52 patients with PXE during follow-up. We found characteristic round or ovoid tubular structures with highly reflective annular lines (termed ‘outer retinal tubulation' (ORT)) in 31 (70.5%) of 44 eyes with CNV, but none were found in eyes without CNV. We also found characteristic undulations of Bruch's membrane in 38 (73.1%) eyes with AS. The incidence of ORT was significantly greater in eyes with CNV secondary to AS (70.8% P=0.005) compared with eyes with CNV secondary to AMD (34.1%). The incidence of Bruch's membrane undulation was significantly greater in eyes with CNV secondary to AS (70.8% P<0.0001) than in eyes with CNV secondary to AMD (11.4%).
Conclusion
SD-OCT imaging clearly revealed a greater incidence of unique lesions, including ORT and Bruch's membrane undulation, in eyes in PXE patients with CNV secondary to AS than in eyes with CNV secondary to AMD.
Keywords: pseudoxanthoma elasticum, angioid streaks, age-related macular degeneration, spectral-domain optical coherence tomography
Introduction
Pseudoxanthoma elasticum (PXE) is a rare systemic disease, affecting 1 in 25 000–100 000 people; it mainly affects the cardiovascular system, skin, and eyes with a variable phenotype.1 This disorder is a consequence of mutations in the ABCC6 gene.2, 3 The progressive fragmentation and calcification of elastic fibres in connective tissue result in pathological changes that are most pronounced in the dermis, Bruch's membrane, and blood vessels.1, 3 Characteristic lesions at the fundus are angioid streaks (AS), peau d'orange, areas of chorioretinal atrophy, and secondary choroidal neovascularization (CNV).1, 3 Although this disorder is rare, it leads to severe vision loss when CNV develops.4, 5
AS occurs as irregular crack-like dehiscence in the Bruch's membrane. These cracks in the Bruch's membrane allow the ingrowth of the CNV into the subretinal space.6 Although AS may occur in isolation or as ocular manifestations of systemic diseases, such as Paget's disease, acromegaly, or Ehlers–Danlos syndrome, the most common disease related to AS is PXE.7, 8, 9
CNVs secondary to AS occur in 72–86% of AS-affected eyes, are bilateral in more than 70% of cases, and tend to affect middle-aged patients. They lead to poor visual prognosis, especially when the CNV involves the macular area.6, 8, 10, 11, 12 The efficacy of various treatment modalities, including laser photocoagulation, transpupillary thermotherapy, PDT with verteporfin, and rarely macular translocation, is limited and even worse in eyes with CNV secondary to AS compared with those secondary to AMD (age-related macular degeneration).7, 9, 13, 14, 15, 16, 17, 18 It is useful to identify the differences in the fundus lesions that underline the different responses to treatment between these diseases.
Spectral-domain optical coherence tomography (SD-OCT) technology allows cross-sectional imaging to an almost histological level by effective reduction of speckle noise, thus allowing the visualization of retinal microstructural abnormalities in vivo.19, 20 Recent studies21, 22 reported unique tomographic features on SD-OCT in eyes with AS, such as breaks in Bruch's membrane, alternating reflectivity within Bruch's membrane associated with Peau d'orange, outer retinal tabulation (ORT),23 and subretinal granular appearance associated with the pattern dystrophy-like changes on fluorescein angiograms and autofluorescence. However, the comparison of SD-OCT findings between eyes with AS and AMD has not been reported.
The purpose of this study is to show the characteristics of AS lesions by comparing SD-OCT features in eyes with CNV secondary to AS associated with PXE with those secondary to AMD by using speckle noise-reduced SD-OCT imaging.
Materials and methods
We conducted a retrospective study of consecutive patients with PXE who were examined by SD-OCT at Kyoto University Hospital from January 2008 to May 2010. All patients underwent complete ophthalmic examination including best-corrected visual acuity using the 5-m Landolt chart, intraocular pressure measurements using a Goldmann applanation tonometer, slit-lamp examinations, fundus examination with colour fundus photography (Topcon TRC50LX; Topcon, Tokyo, Japan), indirect ophthalmoscopy, and slit-lamp biomicroscopy. Fluorescein angiography (FA), indocyanine green angiography, and SD-OCT were performed simultaneously by using a Spectralis HRA+OCT (Heidelberg Engineering, Heidelberg, Germany). Patient data were collected through a review of our medical records. All investigations of this study adhered to the tenets of the Declaration of Helsinki. This study was approved by the Institutional Review Board and Ethics Committee of the Kyoto University Graduate School of Medicine. Informed consent was obtained for all patients.
The diagnosis of PXE was based on characteristic systemic manifestation including fundus findings in photographs as well as FA as described by Shields et al.11 The diagnosis was then further confirmed by characteristic histopathological abnormalities in skin biopsy. Eyes with opaque media (eg, cataracts) and vitreous haemorrhage were excluded from analysis.
SD-OCT imaging
SD-OCT imaging was performed for all patients by using a Spectralis HRA+OCT system with automated eye tracking and image alignment modules. The software allows the averaging of up to 100 single B-scan images from the same location of interest in real time (automatic real-time ‘ART' module). This enables effective speckle noise reduction, which markedly improves the visualization of retinal layer boundaries and the borders of various pathological lesions to help reveal the detailed structures of various pathologies.19, 20, 21, 22
Our routine SD-OCT examination, using this instrument, included 6- or 9-mm radial macular scans, and 6- or 9-mm horizontal and vertical serial scans for the entire macular area. In each scan, 50 B scans were averaged to reduce speckle noise.
Comparative studies of eyes with CNV secondary to AS and AMD
We compared the incidence of characteristic tomographic features between eyes with CNV secondary to AS and AMD. All of the patients with AS who had CNV were included in this comparison. Control eyes with CNV secondary to AMD were randomly selected from patient pools in Kyoto University Hospital from January 2008 to May 2010. When both eyes in a single patient were eligible for this comparison, one eye was selected randomly.
Statistics
Statistical analysis was performed using the SPSS statistical software (version 16; SPSS Inc., Chicago, IL, USA). Fisher's exact test was used to analyse categorical variables (gender, incidence of ORT, cystoid spaces, Bruch's membrane undulation, serous retinal detachment (SRD), and locations of ORT). Unpaired t-tests were used to compare numerical variables (age, refraction, best-corrected visual acuity, SD-OCT follow-up period, and the number of SD-OCT examinations). Best-corrected visual acuity was converted to the logarithm of the minimum angle of resolution (LogMAR) for statistical analysis. P-values less than 0.05 were considered statistically significant.
Results
A total of 52 eyes from 27 patients (17 males and 10 females) were included in this retrospective study. All patients were Japanese (Table 1). Their mean age was 65.8 (SD, 10.1) years (range, 50–89 years). The mean follow-up time was 39.5 (18.8) months (range, 3–61 months). The mean follow-up period by SD-OCT was 12.6 (9.6) months (range, 1–27 months). The mean number of SD-OCT examinations per patient was 4.1 (3.1) (range, 1–13 examinations). Two eyes from two patients were excluded due to the presence of vitreous haemorrhage and cataract in one eye each. Seven eyes with AS were excluded because of poor SD-OCT image quality.
Table 1. Demographics and tomographic features of patients with pseudoxanthoma elasticum.
Demographics | |
---|---|
Number | 52 eyes (27 patients) |
Men/women | 17/10 (62.9%/37.1%) |
Age (years)a | 65.8 (10.1) (range, 50–89) |
Follow-up period (months)a | 39.5 (18.8) (range, 3–61) |
SD-OCT follow-up period (months)a | 12.6 (9.6) (range, 1–27) |
Results of skin biopsy, patient number (%)b | 26 (96.3%) PXE positive(biopsy was not done for one patient) |
Refractiona | −1.3 (2.05) (−6.5 to 1.5) |
Log MAR BCVA at first SD-OCT visita | 0.68 (0.54) (range, −1.8 to 1.6) |
Change in log MAR BCVAa | 0.19 (0.36) (range, −0.33 to 1.3) |
Secondary CNVb | 44 eyes (84.6%) |
Tomographic features | |
ORT (%)b | 31 (59.6%) |
Location of ORT | |
On border of CNV | 23 (74.2%) |
Over CNV | 12 (38.7%) |
Cystoid spacesb | 23 (44.2%) |
Bruch's membrane undulation (%)b | 38 (73.1%) |
SRD (%)b | 7 (13.5%) |
Abbreviations: AS, angioid streaks; BCVA, best-corrected visual acuity; CNV, choroidal neovascularization; Log MAR, logarithm of the minimal angle of resolution; ORT, outer retinal tubulation; PXE, pseudoxanthoma elasticum; SRD, serous retinal detachment; SD-OCT, spectral-domain optical coherence tomography.
Values are expressed as means (SD), with the range shown in adjacent parentheses.
Values are the number of eyes, with percentage shown in parenthesis.
All 27 patients exhibited systemic manifestations of PXE, including characteristic skin and fundus lesions. In all, 26 patients (96.3%) were diagnosed with PXE on the basis of skin biopsy results (skin biopsy was not performed for 1 patient).
Biomicroscopic and angiographic findings
Angioid streaks were seen in all of the 52 eyes, and Peau d'orange were observed in 50 (96.2%) eyes. No eyes showed the pattern dystrophy-like changes on fluorescein angiogram or optic nerve head drusen.
Secondary CNV was found in 44 eyes (84.6%) of 52 eyes with PXE by FA at the initial examination. No other eyes developed CNV during the follow-up as observed by SD-OCT. SRD was only found in 7 eyes (13.5%) when observed using SD-OCT.
Of the 44 eyes with secondary CNV, 26 (59.1%) eyes had a history of treatment for CNV before the beginning of SD-OCT follow-up (successive PDT and anti-VEGF for 14 eyes, anti-VEGF therapy only for 5 eyes, and photodynamic therapy only for 7 eyes) and 16 (36.4%) eyes received treatment during the follow-up by SD-OCT (anti-VEGF therapy only for 8 eyes and combined photodynamic and anti-VEGF therapies for 8).
Tomographic findings in eyes with AS
Outer retinal tabulation
Incidence and morphology: We found that there were characteristic round or ovoid tubular structures in the outer retina in 31 (59.6%) of 52 eyes (Table 1). These structures were nearly same as those reported to be ‘outer retinal tubulation' (ORT) found primarily in eyes with AMD.23 ORT structures were found in 31 (70.5%) of 44 eyes with secondary CNV but were not found in any eye without CNV.
The ORT structures were composed of hyperreflective round/ovoid annular lines (Figure 1). The centres of ORTs were clear or contained highly reflective amorphous materials within the annulus. The vertical diameters of the highly reflective annulus in the SD-OCT images ranged from 68 to 125 μm. The horizontal diameters appeared to be highly variable because they depend on the direction of the OCT scan.
Serial SD-OCT images revealed that the ORT structures formed round structures or short segments in many cases, whereas they formed branching tubular morphology in others. The simultaneous acquisition of FA and SD-OCT images further showed that the some ORTs in the serial SD-OCT images were well correlated with the staining during the late phase of FA that was observed within the CNV in short segments or branching tubule patterns (Figure 2). At the early phase of FA, these tubular structures did not exhibit dye filling as seen in the retinal and choroidal vessels.
Location and photoreceptor damage: The ORT structures appeared to be located beneath the outer plexiform layer (OPL) because they were observed beneath the highly reflective line representing the OPL (Figure 1).
The extent of the ORT structures was determined in relation to the location of the CNV; ORT structures were found at the border of and over the CNV lesions in 23 (74.2%) and 12 (38.7%) of 31 eyes, respectively (Figure 1). However, the ORT was commonly located within the areas where the photoreceptor layer appeared degenerated or in the vicinity of the junction between healthy and degenerated photoreceptor layer. Regardless of the location of the ORT in relation to the CNV, few features representing the integrity of photoreceptor layers were observed, such as the external limiting membrane and the photoreceptor inner and outer segment junction beneath the ORT (Figure 1).
Temporal changes: ORT structures were found before and after anti-CNV treatment in all eyes with AS and secondary CNV that received treatment (26 eyes) during the SD-OCT follow-up. During the follow-up period with serial SD-OCT, ORT structures appeared to remain stable even after anti-CNV treatment.
ORT vs cystoid spaces: Both ORTs and cystoid spaces appeared to be round in shape in SD-OCT images. However, the tomographic features of ORTs and cystoid spaces in speckle noise-reduced SD-OCT images were different. The ORT structures had highly reflective annular margins and were located beneath the OPL hyperreflective band, whereas no cystoid spaces had hyperreflective annular margins and large parts of them were located in the inner nuclear layer or the OPL. Simultaneous imaging with FA revealed that the cystoid spaces corresponded to active dye filling in a petaloid or cystic pattern, whereas the ORT structures corresponded to faint staining in short segments or in a branching tubular pattern. Based on the FA images, 23 eyes with AS exhibited cystoid spaces, 13 had a petaloid pattern characteristic to cystoid macular edema, and 10 had cystoid spaces without a petaloid pattern.
Bruch's membrane undulation: SD-OCT imaging enabled us to observe the morphology of Bruch's membrane, particularly in the areas with retinal pigment epithelium degeneration where the remaining Bruch's membrane was directly observable as a highly reflective line (Figure 3). The architecture of the Bruch's membrane in eyes with CNV secondary to AS was unique; the Bruch's membrane did not only exhibit disruption corresponding to the AS in colour fundus photographs but also exhibited repetitively inward and outward deformation, giving it a ‘wavy' appearance. We term the former feature ‘Bruch's membrane disruptions', and the latter ‘Bruch's membrane undulations'. Bruch's membrane undulations were not detected by other imaging modalities including FA, indocyanine green angiography, autofluorescence, and near-infrared reflectance. Bruch's membrane undulations were found in 38 (73.1%) out of a total of 52 eyes, 35 (79.5%) of 44 eyes with CNV and only in 3 (37.5%) of 8 eyes without CNV (Table 1). All of the Bruch's membrane undulations were found beneath the CNV in the macular regions. Some were located within areas with CNV, whereas others partially extended outside those areas. Disruptions in Bruch's membrane, which were found in all eyes with AS, were also detected within the areas with CNV on the SD-OCT images.
Comparison of the incidence of tomographic features between eyes with CNV secondary to AS and AMD
The incidence of characteristic tomographic features was compared between 24 eyes from 24 patients with CNV secondary to AS and 44 eyes from 44 patients with CNV secondary to AMD. The demographics of the patients are summarized in Table 2. The patients with CNV secondary to AS were significantly younger (P<0.0001) and more myopic (P=0.027). There were no significant differences in gender or the logarithm of the minimal angle of resolution best-corrected visual acuity.
Table 2. Comparison between eyes with choroidal neovascularization secondary to angioid streaks and age-related macular degeneration.
AS | AMD | P-value | |
---|---|---|---|
Demographics | |||
Number | 24 eyes (24 patients) | 44 eyes (44 patients) | |
Age (years)a | 66.8 (9.4) (50–89) | 75.3 (6.5) (62–85) | <0.0001b |
Men/women | 16/8 (66.7%/33.3%) | 29/15 (65.9%/34.1%) | 0.789c |
Refraction (dioptres)a | −1.2 (2.15) (−6.75 to 1.5) | 0.1 (1.96) (−5.5 to 2.0) | 0.027b |
Log MAR BCVA at first SD-OCT visita | 0.747 (0.47) (−0.08 to 1.39) | 0.69 (0.46) (−0.08 to 1.70) | 0.565b |
Tomographic features | |||
SD-OCT follow-up period (months)a | 13.5 (9.1) (1–27) | 11.6 (9.2) (1–29) | 0.436 |
Number. of SD-OCT examinationsa | 4.1 (2.9) (1–13) | 4.0 (3.0) (1–11) | 0.480b |
ORT (%)d | 17 (70.8%) | 15 (34.1%) | 0.005c |
Location of ORT | |||
On border of CNV | 13 (76.5%) | 5 (33.3%) | 0.031c |
Over CNV | 6 (35.3%) | 11 (73.3%) | 0.042c |
Cystoid spaces (%)d | 14 (58.3%) | 26 (59.1%) | 0.952c |
Bruch's membrane undulation (%)d | 17 (70.8%) | 5 (11.4%) | <0.0001c |
SRD (%)d | 4 (16.7%) | 26 (59.1%) | 0.001c |
PED (%)d | 0 | 6 (13.6%) |
Abbreviations: AMD, age-related macular degeneration; Log MAR, logarithm of the minimal angle of resolution; No, number; ORT, outer retinal tubulation; SD-OCT, spectral-domain optical coherence tomography; SRD, serous retinal detachment; PED, pigment epithelial detachment.
Values are expressed as means (SD).
Unpaired t-test.
Fisher's exact test.
Values are the number of eyes, with percentage shown in parenthesis.
The results of the tomographic features are summarized in Table 2. The follow-up duration and number of SD-OCT examinations were not significantly different between groups. During the SD-OCT follow-up, the incidence of ORT was significantly greater in eyes with CNV secondary to AS (70.8% P=0.005) than in eyes with CNV secondary to AMD (34.1%). In the AS group, ORTs were more frequently observed on the border of the CNV in SD-OCT images (76.5%, P=0.031) compared with those in the AMD group (33.3%). In contrast, ORTs were less frequently observed over the CNV in the AS group (35.3%, P=0.042) compared with those in the AMD group (73.3%). The incidence of Bruch's membrane undulation was significantly greater in eyes with CNV secondary to AS (70.8% P<0.0001) than in eyes with CNV secondary to AMD (11.4%). The features of Bruch's membrane undulation in the SD-OCT images were qualitatively different between groups; the waving pattern in the Bruch's membrane was apparent in the AS group but minimal in the AMD group (Figure 3). We found SRDs only in 16.7% of eyes with CNV secondary to AS but in 59.1% eyes with CNV secondary to AMD; this difference is statistically significant (P<0.001). Moreover, the SRDs in the AS group appear to be shallow compared with those in the AMD group. Cystoid spaces were found in 14 (58.3%) of 24 eyes with CNV secondary to AS and in 26 (59.1%) of 44 eyes with CNV secondary to AMD; this difference was not statistically significant (Table 2). No pigment epithelial detachment (PED) was found in eyes with CNV secondary to AS, whereas serous PED was found in 6 (13.6%) of 44 eyes with CNV secondary to AMD (Table 2).
Discussion
In the current retrospective study, we found that, in eyes with CNV secondary to AS, ORTs were more frequently seen and Bruch's membrane undulations were more commonly observed, compared with eyes with CNV secondary to AMD. The incidence of SRD was lower and PED was not seen in eyes with CNV secondary to AS. These differences in tomographic features appear to be useful in facilitating our understanding of the different pathologies between PXE and AMD.
Zweifel et al23 proposed the term ‘outer retinal tubulation' (ORT) to describe round- or ovoid-shaped structures on SD-OCT images mainly observed in eyes with AMD. In the present study, the ORT structures found in eyes with PXE were similar to those described in their study in that they had similar round or ovoid appearances with highly reflective annular lines, similar localization in the ONL, and stability over time, even after treatment.
They showed that the incidence of ORT in eyes with AMD was 24.2%.23 The same authors reported in another report that the ORT was seen in 17 (40.5%) of 42 AS eyes.22 It is noted that, in the latter study, only 7 (16.7%) eyes had evidence of CNV, indicating that ORT can occur in AS eyes without CNV. In our study, the incidence of ORTs was high (70.8%) in AS eyes with CNV; this incidence was much higher than (34.1%) that in AMD eyes with CNV. This higher incidence of ORT in AS eyes compared with AMD eyes is consistent with the previous studies.22, 23 However, we did not find any ORT structures in AS eyes without CNV in our subjects, inconsistent with the previous studies.22, 23 It is possible that the AS subjects differed with stages and race between studies. In fact, although the previous studies reported that the pattern dystrophy-like changes on fluorescein angiogram and the optic nerve head drusen were observed in a part of study eyes,21, 22 we did not find these fundus features in any subjects. It is uncertain whether the much higher incidence of CNV in our study eyes compared with the previous studies is responsible for some of these differences. The absence of ORT in AS eyes without CNV cannot be fully explained by the high incidence of CNV. Another possibility is that the genetic differences with race causes the variability of the disease phenotype; if this is true, further studies are needed to demonstrate racial differences of fundus features associated with the PXE.
In our study, ORT structures were most frequently found in the vicinity of and over the CNV border in the PXE and AMD groups, respectively. Also, in some eyes, ORTs were observed as faint staining within the CNV during the late phase of FA. Zweifel et al23 speculated that ORT represents a misguided reparative attempt of the photoreceptors and their processes in a ‘circling of the wagons' phenomenon. The current study showed the greater incidence of ORT structures and their more frequent localization in the vicinity of the border of the CNV in eyes with AS compared with those with AMD may stem from some of the differences of CNV development between these two diseases, for example, with respect to the severity, area, and speed of photoreceptor layer damage.
Rosette formation is reported in various animal models, including neural retina leucine zipper (Nrl) knockout and Pax6 overexpression mice, and in models of retina transplantation.24, 25, 26, 27 In the retina of Nrl knockout mice, Fischer et al24 show that the histology of rosette structures corresponds with the round or ovoid features with highly reflective annular lines observed in speckle noise-reduced SD-OCT. Although it remains unclear whether the rosettes in animal models are same as the ORT in human patients,26 this correspondence suggests that the ORT is comprised of rearranged neuronal cells. Unfortunately, histopathological evidence that supports the presence and contents of ORT structures in patients' eyes is limited. We found rosette structures in eyes with AS and AMD in a few light microscope sections.28 These structures are actually comprised of circularly arranged photoreceptors containing a single or a few cells inside and were observed immediately anterior to the retinal pigment epithelium. Taken together, the interpretation of the ORT as a photoreceptor rosette appears to be appropriate.
Bruch's membrane undulation appears to be characteristic in eyes with CNV secondary to AS because it occurred six times more frequently compared with eyes with CNV secondary to AMD. In AMD, Bruch's membrane undulations were, if present at all, less prominent. In their presentation of the tomographic features of patients with PXE, Charbel Issa et al21 focused on the variable disruptions of the Bruch's membrane, including smaller and larger breaks with displaced edges or fibrous tissue growing within it, as well as its degradation into pieces. In our study, although we found similar variable disruptions and degradation of the Bruch's membrane, we additionally focused on the undulations because this feature appears to be closely associated with the underlying causes of PXE lesions; progressive fragmentation and calcification of the elastic fibres in the Bruch's membrane appear to make it weak.1, 2 The undulations likely represent the weak integrity of the Bruch's membrane.
The wavy architecture, breaks, and the dislocation of fragmented pieces of the Bruch's membrane have been shown in previous histopathological studies.28, 29 Although it was unclear whether these minute pathologies are real, or artifacts resulting from postmortem changes, and histological fixation, noninvasive SD-OCT imaging revealed that this characteristic feature of the Bruch's membrane are in fact a real finding.
In our subjects, the incidence of SRDs was significantly lower in eyes with CNV secondary to AS (15.9%) compared with eyes with CNV secondary to AMD (54.5%) during follow-up by SD-OCT. Ozawa et al30 found SRDs in 53% of eyes with exudative AMD. Zweifel et al22 found SRDs in 33.3% of AS eyes; they showed that the SRDs were not only associated with leakage from CNV, but also with pattern dystrophy-like findings. As mentioned above, pattern dystrophy-like findings were not observed in our study. Thus, active leakage from CNV may less frequently occur in AS eyes compared with AMD eyes.
A limitation of this study is the variable subject conditions, particularly therapeutic conditions, which stem from its retrospective cohort design. Another limitation is that our study included only Japanese subjects, which may limit the interpretation of the results. However, this limitation provides a simpler study population not affected by ethnic differences, which may be beneficial for comparing SD-OCT features between AS eyes and AMD eyes.
Conclusion
In conclusion, speckle noise-reduced SD-OCT imaging clearly revealed a high incidence of ORTs and Bruch's membrane undulation in Japanese eyes with PXE. The incidence of these features was much higher in AS eyes with CNV than AMD eyes with CNV; the incidence of SRD was also much lower. Thus, SD-OCT imaging allows the characterization of unique fundus lesions characteristic to AS.
Masanori Hangai is a paid advisory board member for NIDEK, and received consulting fees from Topcon, lecture fees from Heidelberg Engineering and Santen. Yoshimura Nagahisa is a paid advisory board member for NIDEK, lecture fees from Nidek, Topcon, and Canon, and research funding from Topcon, Nidek, and Canon. The other authors declare no conflict of interest.
References
- Hu X, Plomp AS, van Soest S, Wijnholds J, de Jong PT, Bergen AA. Pseudoxanthoma elasticum: a clinical, histopathological, and molecular update. Surv Ophthalmol. 2003;48:424–438. doi: 10.1016/s0039-6257(03)00053-5. [DOI] [PubMed] [Google Scholar]
- Le Saux O, Urban Z, Tschuch C, Csiszar K, Bacchelli B, Quaglino D, et al. Mutations in a gene encoding an ABC transporter cause pseudoxanthoma elasticum. Nat Genet. 2000;25:223–227. doi: 10.1038/76102. [DOI] [PubMed] [Google Scholar]
- Finger RP, Charbel Issa P, Ladewig MS, Gotting C, Szliska C, Scholl HP, et al. Pseudoxanthoma elasticum: genetics, clinical manifestations and therapeutic approaches. Surv Ophthalmol. 2009;54:272–285. doi: 10.1016/j.survophthal.2008.12.006. [DOI] [PubMed] [Google Scholar]
- Mimoun G, Tilleul J, Leys A, Coscas G, Soubrane G, Souied EH. Intravitreal ranibizumab for choroidal neovascularization in angioid streaks. Am J Ophthalmol. 2010;150:692–700. doi: 10.1016/j.ajo.2010.06.004. [DOI] [PubMed] [Google Scholar]
- Sawa M, Gomi F, Tsujikawa M, Sakaguchi H, Tano Y. Long-term results of intravitreal bevacizumab injection for choroidal neovascularization secondary to angioid streaks. Am J Ophthalmol. 2009;148:584–590. doi: 10.1016/j.ajo.2009.04.026. [DOI] [PubMed] [Google Scholar]
- Dreyer R, Green WR. The pathology of angioid streaks: a study of twenty-one cases. Trans Pa Acad Ophthalmol Otolaryngol. 1978;31:158–167. [PubMed] [Google Scholar]
- Clarkson JG, Altman RD. Angioid streaks. Surv Ophthalmol. 1982;26:235–246. doi: 10.1016/0039-6257(82)90158-8. [DOI] [PubMed] [Google Scholar]
- Connor PJ, Juergens JL, Perry HO, Hollenhorst RW, Edwards JE. Pseudoxanthoma elasticum and angioid streaks. A review of 106 cases. Am J Med. 1961;30:537–543. doi: 10.1016/0002-9343(61)90078-x. [DOI] [PubMed] [Google Scholar]
- Dabbs TR, Skjodt K. Prevalence of angioid streaks and other ocular complications of Paget's disease of bone. Br J Ophthalmol. 1990;74:579–582. doi: 10.1136/bjo.74.10.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mansour AM, Shields JA, Annesley JrWH, el-Baba F, Tasman W, Tomer TL. Macular degeneration in angioid streaks. Ophthalmologica. 1988;197:36–41. doi: 10.1159/000309915. [DOI] [PubMed] [Google Scholar]
- Shields JA, Federman JL, Tomer TL, Annesley WH. Angioid streaks. I. Ophthalmoscopic variations and diagnostic problems. Br J Ophthalmol. 1975;59:257–266. doi: 10.1136/bjo.59.5.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lim JI, Bressler NM, Marsh MJ, Bressler SB. Laser treatment of choroidal neovascularization in patients with angioid streaks. Am J Ophthalmol. 1993;116:414–423. doi: 10.1016/s0002-9394(14)71398-4. [DOI] [PubMed] [Google Scholar]
- Brancato R, Menchini U, Pece A, Davi G, Capoferri C. Laser treatment of macular subretinal neovascularizations in angioid streaks. Ophthalmologica. 1987;195:84–87. doi: 10.1159/000309793. [DOI] [PubMed] [Google Scholar]
- Ozdek S, Bozan E, Gurelik G, Hasanreisoglu B. Transpupillary thermotherapy for the treatment of choroidal neovascularization secondary to angioid streaks. Can J Ophthalmol. 2007;42:95–100. [PubMed] [Google Scholar]
- Aras C, Baserer T, Yolar M, Yetik H, Artunay O, Guzel H, et al. Two cases of choroidal neovascularization treated with transpupillary thermotherapy in angioid streaks. Retina. 2004;24:801–803. doi: 10.1097/00006982-200410000-00020. [DOI] [PubMed] [Google Scholar]
- Bhatnagar P, Freund KB, Spaide RF, Klancnik JrJM, Cooney MJ, Ho I, et al. Intravitreal bevacizumab for the management of choroidal neovascularization in pseudoxanthoma elasticum. Retina. 2007;27:897–902. doi: 10.1097/IAE.0b013e31809ff5df. [DOI] [PubMed] [Google Scholar]
- Karacorlu M, Karacorlu S, Ozdemir H, Mat C. Photodynamic therapy with verteporfin for choroidal neovascularization in patients with angioid streaks. Am J Ophthalmol. 2002;134:360–366. doi: 10.1016/s0002-9394(02)01626-4. [DOI] [PubMed] [Google Scholar]
- Menchini U, Virgili G, Introini U, Bandello F, Ambesi-Impiombato M, Pece A, et al. Outcome of choroidal neovascularization in angioid streaks after photodynamic therapy. Retina. 2004;24:763–771. doi: 10.1097/00006982-200410000-00013. [DOI] [PubMed] [Google Scholar]
- Sakamoto A, Hangai M, Yoshimura N. Spectral-domain optical coherence tomography with multiple B-scan averaging for enhanced imaging of retinal diseases. Ophthalmology. 2008;115:1071–1078. doi: 10.1016/j.ophtha.2007.09.001. [DOI] [PubMed] [Google Scholar]
- Hangai M, Yamamoto M, Sakamoto A, Yoshimura N. Ultrahigh-resolution versus speckle noise-reduction in spectral-domain optical coherence tomography. Opt Express. 2009;17:4221–4235. doi: 10.1364/oe.17.004221. [DOI] [PubMed] [Google Scholar]
- Charbel Issa P, Finger RP, Holz FG, Scholl HP. Multimodal imaging including spectral domain OCT and confocal near infrared reflectance for characterization of outer retinal pathology in pseudoxanthoma elasticum. Invest Ophthalmol Vis Sci. 2009;50:5913–5918. doi: 10.1167/iovs.09-3541. [DOI] [PubMed] [Google Scholar]
- Zweifel SA, Imamura Y, Freund KB, Spaide RF. Multimodal fundus imaging of pseudoxanthoma elasticum. Retina. 2011;31:482–491. doi: 10.1097/IAE.0b013e3181f056ce. [DOI] [PubMed] [Google Scholar]
- Zweifel SA, Engelbert M, Laud K, Margolis R, Spaide RF, Freund KB. Outer retinal tubulation: a novel optical coherence tomography finding. Arch Ophthalmol. 2009;127:1596–1602. doi: 10.1001/archophthalmol.2009.326. [DOI] [PubMed] [Google Scholar]
- Fischer MD, Huber G, Beck SC, Tanimoto N, Muehlfriedel R, Fahl E, et al. Noninvasive, in vivo assessment of mouse retinal structure using optical coherence tomography. PLoS One. 2009;4:e7507. doi: 10.1371/journal.pone.0007507. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wenzel A, von Lintig J, Oberhauser V, Tanimoto N, Grimm C, Seeliger MW. RPE65 is essential for the function of cone photoreceptors in NRL-deficient mice. Invest Ophthalmol Vis Sci. 2007;48:534–542. doi: 10.1167/iovs.06-0652. [DOI] [PubMed] [Google Scholar]
- Nikonov SS, Daniele LL, Zhu X, Craft CM, Swaroop A, Pugh EN. Photoreceptors of Nrl -/- mice coexpress functional S- and M-cone opsins having distinct inactivation mechanisms. J Gen Physiol. 2005;125:287–304. doi: 10.1085/jgp.200409208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Daniele LL, Lillo C, Lyubarsky AL, Nikonov SS, Philp N, Mears AJ, et al. Cone-like morphological, molecular, and electrophysiological features of the photoreceptors of the Nrl knockout mouse. Invest Ophthalmol Vis Sci. 2005;46:2156–2167. doi: 10.1167/iovs.04-1427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green WR. Ophthalmic Pathology: An Atlas and Textbook. Saunders: Philadelphia; 1996. [Google Scholar]
- Hagedoorn A. Angioid streaks. Arch Ophthalmol. 1939;21:746–774. [Google Scholar]
- Ozawa S, Ishikawa K, Ito Y, Nishihara H, Yamakoshi T, Hatta Y, et al. Differences in macular morphology between polypoidal choroidal vasculopathy and exudative age-related macular degeneration detected by optical coherence tomography. Retina. 2009;29:793–802. doi: 10.1097/IAE.0b013e3181a3b7d9. [DOI] [PubMed] [Google Scholar]