Correction for Rottlaender et al., Glycogen synthase kinase 3β transfers cytoprotective signaling through connexin 43 onto mitochondrial ATP-sensitive K⁺ channels

MEDICAL SCIENCES Correction for “Glycogen synthase kinase 3β transfers cytoprotective signaling through connexin 43 onto mitochondrial ATP-sensitive K⁺ channels,” by Dennis Rottlaender, Kerstin Boengler, Martin Wolny, Astrid Schwaiger, Lukas J. Motloch, Michel Ovize, Rainer Schulz, Gerd Heusch, and Uta C. Hoppe, which appeared in issue 5, January 31, 2012, of Proc Natl Acad Sci USA (109:E242–E251; first published January 11, 2012; 10.1073/pnas.1107479109).

The authors note that some baseline traces in Fig. 1 appeared incorrectly. The corrected figure and its legend appear below. This error does not affect the conclusions of the article.

Fig. 1.

MitoK_ATP single-channel activation by the GSK3β inhibitor SB216763 (5 μM) is mediated via Cx43. (A) Baseline (Left) and SB216763-activated (Center) mitoK_ATP currents of WT mitoplasts, which were blocked by 100 μM MgATP (Right) (at −60 mV). (B) Mean values of Po,total of WT mitoplasts without (black) and with (gray, 10μM) carbenoxolone or ⁴³GAP27 (white, 250 µM) and in mitoplasts from Cx43^Cre-ER(T)/fl + 4-OHT mice (stripes) in the absence and presence of SB216763 and MgATP, as indicated (N values in parentheses). *P < 0.05 vs. control; ^#P < 0.05 vs. control + SB216763. (C) Single-channel amplitude (i) as a function of test potentials (V_m). Slope conductances determined by linear regression in individual experiments of WT mitoplasts were unaffected by SB216763 with or without MgATP compared with control. (D) Baseline single-channel properties were unaffected (Left), whereas the SB216763 effect on mitoK_ATP channel activity was reduced in the presence of 10 μM carbenoxolone (Center) (at −60 mV) compared with control (A). (Right) Channel inhibition by MgATP. (E) Baseline single-channel properties were unaffected (Left), whereas the SB216763 effect on mitoK_ATP channel activity was reduced in the presence of 250 µM ⁴³GAP27 (Center) (at −60 mV) compared with control (A). (Right) Channel inhibition by MgATP. (F) SB216763 effect on mitoK_ATP channel activity was almost abolished in Cx43^Cre-ER(T)/fl + 4-OHT mitoplasts. (Right) Channel inhibition by MgATP.