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. 1978 Apr;5(4):1207–1219. doi: 10.1093/nar/5.4.1207

Nucleotide sequences at the 5' termini of influenza virus RNAs and their transcripts.

J J Skehel, A J Hay
PMCID: PMC342071  PMID: 652519

Abstract

The results of analyses of the 5'-terminal sequences of Fowl Plague virus RNAs are presented. The first 13 residues of each of the eight RNA molecules which constitute the genome are in the identical sequence 5'AGUAGAAAUUAGG- and this conservation of sequence is shown to extend to other influenza viruses. The 5'-terminal sequences of virion RNA transcripts produced in vitro are also reported and again the first 12 nucleotides of these are identical for all influenza type A transcripts examined in the sequence 5'AGCAAAAGCAGG-. In addition the results of attempts to determine the sequence relationship between vRNAs and the two classes of complementary RNA synthesized in influenza infected cells are described which support the conclusion that influenza messenger RNAs are incomplete transcripts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dasgupta R., Kaesberg P. Sequence of an oligonucleotide derived from the 3' end of each of the four brome mosaic viral RNAs. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4900–4904. doi: 10.1073/pnas.74.11.4900. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Etkind P. R., Buchhagen D. L., Herz C., Broni B. B., Krug R. M. The segments of influenza viral mRNA. J Virol. 1977 May;22(2):346–352. doi: 10.1128/jvi.22.2.346-352.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Etkind P. R., Krug R. M. Influenza viral messenger RNA. Virology. 1974 Nov;62(1):38–45. doi: 10.1016/0042-6822(74)90301-8. [DOI] [PubMed] [Google Scholar]
  5. Glass S. E., McGeoch D., Barry R. D. Characterization of the mRNA of influenza virus. J Virol. 1975 Dec;16(6):1435–1443. doi: 10.1128/jvi.16.6.1435-1443.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hay A. J., Abraham G., Skehel J. J., Smith J. C., Fellner P. Influenza virus messenger RNAs are incomplete transcripts of the genome RNAs. Nucleic Acids Res. 1977 Dec;4(12):4197–4209. doi: 10.1093/nar/4.12.4197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hay A. J., Lomniczi B., Bellamy A. R., Skehel J. J. Transcription of the influenza virus genome. Virology. 1977 Dec;83(2):337–355. doi: 10.1016/0042-6822(77)90179-9. [DOI] [PubMed] [Google Scholar]
  8. Hay A. J. Studies on the formation of the influenza virus envelope. Virology. 1974 Aug;60(2):398–418. doi: 10.1016/0042-6822(74)90335-3. [DOI] [PubMed] [Google Scholar]
  9. Kozak M. Nucleotide sequences of 5'-terminal ribosome-protected initiation regions from two reovirus messages. Nature. 1977 Sep 29;269(5627):391–394. doi: 10.1038/269390a0. [DOI] [PubMed] [Google Scholar]
  10. McGeoch D., Fellner P., Newton C. Influenza virus genome consists of eight distinct RNA species. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3045–3049. doi: 10.1073/pnas.73.9.3045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McGeoch D., Kitron N. Influenza virion RNA-dependent RNA polymerase: stimulation by guanosine and related compounds. J Virol. 1975 Apr;15(4):686–695. doi: 10.1128/jvi.15.4.686-695.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Plotch S. J., Krug R. M. Influenza virion transcriptase: synthesis in vitro of large, polyadenylic acid-containing complementary RNA. J Virol. 1977 Jan;21(1):24–34. doi: 10.1128/jvi.21.1.24-34.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RALPH R. K., BELLAMY A. R. ISOLATION AND PURIFICATION OF UNDEGRADED RIBONUCLEIC ACIDS. Biochim Biophys Acta. 1964 May 18;87:9–16. doi: 10.1016/0926-6550(64)90041-6. [DOI] [PubMed] [Google Scholar]
  14. Ritchey M. B., Palese P., Kilbourne E. D. RNAs of influenza A, B, and C viruses. J Virol. 1976 May;18(2):738–744. doi: 10.1128/jvi.18.2.738-744.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rose J. K. Nucleotide sequences of ribosome recgonition sites in messenger RNAs of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3672–3676. doi: 10.1073/pnas.74.9.3672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Scholtissek C., Harms E., Rohde W., Orlich M., Rott R. Correlation between RNA fragments of fowl plague virus and their corresponding gene functions. Virology. 1976 Oct 15;74(2):332–344. doi: 10.1016/0042-6822(76)90340-8. [DOI] [PubMed] [Google Scholar]
  17. Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
  18. Skehel J. J., Hay A. J. Influenza virus transcription. J Gen Virol. 1978 Apr;39(1):1–8. doi: 10.1099/0022-1317-39-1-1. [DOI] [PubMed] [Google Scholar]

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