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. 1978 Apr;5(4):1273–1287. doi: 10.1093/nar/5.4.1273

On the biosynthesis of 5-methoxyuridine and uridine-5-oxyacetic acid in specific procaryotic transfer RNAs

K Murao 1, H Ishikura 1, M Albani 1, H Kersten 1
PMCID: PMC342075  PMID: 418384

Abstract

The uridine-5-0-derivatives, 5-methoxyuridine (mo5U) and uridine-5-oxyacetic acid (cmo5U) occupy the first position of anticodons in certain tRNA species of B. subtilis and E. coli, respectively. Here we present experimental evidence showing that both modifications are derived from a common precursor, 5-hydroxyuridine. Incompletely modified tRNAAla, tRNAThr and tRNAVal were purified from B. subtilis, and submodified tRNASer and tRNAVal from E. coli met− rel−. All five tRNAs accepted methyl groups from S-adenosylmethionine with B. subtilis extracts in vitro and mo5U was formed. In B. subtilis tRNAs the mo5U was proved to be at the specific site; in. E. coli tRNAVal the mo5U was demonstrated to be present in the oligonucleotide that comprises the anticodon. In submethylated E. coli tRNAVal, 5-hydroxyuridine was detected whereas considerable amounts of cmo5U were lacking.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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