Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1978 Apr;5(4):1289–1295. doi: 10.1093/nar/5.4.1289

Initiation of adenovirus DNA replication does not occur via a hairpin mechanism.

J S Sussenbach, M G Kujik
PMCID: PMC342076  PMID: 148639

Abstract

Models have been proposed suggesting that initiation of adenovirus DNA replication might occur via a hairpin mechanism. A consequence of the models is a covalent linkage of progeny and parental DNA in newly completed molecules. Analysis of mature molecules from KB cells infected with adenovirus type 5 indicates that, if a hairpin mechanism is involved, the length of the hairpin must be shorter than 50 basepairs long. Recent nucleotide sequence analysis of the termini of adenovirus type 5 DNA (P.H.Steenbergh et al. (1977) Nucl. Acids Res. 4, 4371-4389) has shown that a hairpin of this size does not exist and that therefore a hairpin mechanism is unlikely.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cavalier-Smith T. Palindromic base sequences and replication of eukaryote chromosome ends. Nature. 1974 Aug 9;250(5466):467–470. doi: 10.1038/250467a0. [DOI] [PubMed] [Google Scholar]
  2. Horwitz M. S. Bidirectional replication of adenovirus type 2 DNA. J Virol. 1976 Apr;18(1):307–315. doi: 10.1128/jvi.18.1.307-315.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kiger J. A., Jr, Sinsheimer R. L. Vegetative lambda DNA. IV. Fractionation of replicating lambda DNA on benzoylated-naphthoylated DEAE cellulose. J Mol Biol. 1969 Mar 28;40(3):467–490. doi: 10.1016/0022-2836(69)90166-1. [DOI] [PubMed] [Google Scholar]
  4. Levine A. J., van der Vliet P. C., Sussenbach J. S. The replication of papovavirus and adenovirus DNA. Curr Top Microbiol Immunol. 1976;73:67–124. doi: 10.1007/978-3-642-66306-2_3. [DOI] [PubMed] [Google Scholar]
  5. Rekosh D. M., Russell W. C., Bellet A. J., Robinson A. J. Identification of a protein linked to the ends of adenovirus DNA. Cell. 1977 Jun;11(2):283–295. doi: 10.1016/0092-8674(77)90045-9. [DOI] [PubMed] [Google Scholar]
  6. Roberts R. J., Arrand J. R., Keller W. The length of the terminal repetition in adenovirus-2 DNA. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3829–3833. doi: 10.1073/pnas.71.10.3829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Schilling R., Weingärtner B., Winnacker E. L. Adenovirus type 2 DNA replication. II. Termini of DNA replication. J Virol. 1975 Oct;16(4):767–774. doi: 10.1128/jvi.16.4.767-774.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Steenbergh P. H., Maat J., van Ormondt H., Sussenbach J. S. The nucleotide sequence at the termini of adenovirus type 5 DNA. Nucleic Acids Res. 1977 Dec;4(12):4371–4389. doi: 10.1093/nar/4.12.4371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Stillman B. W., Bellett A. J., Robinson A. J. Replication of linear adenovirus DNA is not hairpin-primed. Nature. 1977 Oct 20;269(5630):723–725. doi: 10.1038/269723a0. [DOI] [PubMed] [Google Scholar]
  10. Sussenbach J. S., Kuijk M. G. Studies on the mechanism of replication of adenovirus DNA. V. The location of termini of replication. Virology. 1977 Mar;77(1):149–157. doi: 10.1016/0042-6822(77)90414-7. [DOI] [PubMed] [Google Scholar]
  11. Sussenbach J. S., van der Vliet P. C., Ellens D. J., Jansz H. S. Linear intermediates in the replication of adenovirus DNA. Nat New Biol. 1972 Sep 13;239(89):47–49. [PubMed] [Google Scholar]
  12. Tolun A., Pettersson U. Termination sites for adenovirus type 2 DNA replication. J Virol. 1975 Oct;16(4):759–766. doi: 10.1128/jvi.16.4.759-766.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Watson J. D. Origin of concatemeric T7 DNA. Nat New Biol. 1972 Oct 18;239(94):197–201. doi: 10.1038/newbio239197a0. [DOI] [PubMed] [Google Scholar]
  14. Weingärtner B., Winnacker E. L., Tolun A., Pettersson U. Two complementary strand-specific termination sites for adenovirus DNA replication. Cell. 1976 Oct;9(2):259–268. doi: 10.1016/0092-8674(76)90117-3. [DOI] [PubMed] [Google Scholar]
  15. Wu M., Roberts R. J., Davidson N. Structure of the inverted terminal repetition of adenovirus type 2 DNA. J Virol. 1977 Feb;21(2):766–777. doi: 10.1128/jvi.21.2.766-777.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. van der Eb A. J. Intermediates in type 5 adenovirus DNA replication. Virology. 1973 Jan;51(1):11–23. doi: 10.1016/0042-6822(73)90361-9. [DOI] [PubMed] [Google Scholar]
  17. van der Vliet P. C., Sussenbach J. S. The mechanism of adenovirus-DNA synthesis in isolated nuclei. Eur J Biochem. 1972 Nov 7;30(3):584–592. doi: 10.1111/j.1432-1033.1972.tb02130.x. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES