Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1978 Jun;5(6):1767–1777. doi: 10.1093/nar/5.6.1767

Construction of recombinant plasmid carrying the lambda DNA fragment responsible for prophage integration.

N Strizhov, L Tikhomirova
PMCID: PMC342124  PMID: 353729

Abstract

The recombinant DNA molecules were constructed from plasmid RSF2124 and the EcoRI fragment of lambda DNA containing the genes responsible for prophage integration. The presence of these genes in recombinant plasmids was detected genetically. lambda int-gene was shown to be expressed in either orientation of insertion in the plasmid. We found that recombinant plasmid was able to integrate into chromosome of lambda lysogens. The integration of plasmid into host chromosome was demonstrated by contransduction of chromosome and plasmid markers using generalized transducer P1 and by specialized transduction with lambda phages.

Full text

PDF
1767

Images in this article

1771Image
on p.1771
1772Image
on p.1772

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Enquist L. W., Weisberg R. A. The red plaque test: a rapid method for identification of excision defective variants of bacteriophage lambda. Virology. 1976 Jul 1;72(1):147–153. doi: 10.1016/0042-6822(76)90319-6. [DOI] [PubMed] [Google Scholar]
  2. Gottesman M. E., Yarmolinsky M. B. Integration-negative mutants of bacteriophage lambda. J Mol Biol. 1968 Feb 14;31(3):487–505. doi: 10.1016/0022-2836(68)90423-3. [DOI] [PubMed] [Google Scholar]
  3. Guarneros G., Echols H. New mutants of bacteriophage lambda with a specific defect in excision from the host chromosome. J Mol Biol. 1970 Feb 14;47(3):565–574. doi: 10.1016/0022-2836(70)90323-2. [DOI] [PubMed] [Google Scholar]
  4. Helinski D. R., Herschman H. R. Effect of Rec mutations on the activity of colicinogenic factors. J Bacteriol. 1967 Sep;94(3):700–706. doi: 10.1128/jb.94.3.700-706.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ihler G., Kawai Y. Alternate fates of the complementary strands of lambda DNA after infection of Escherichia coli. J Mol Biol. 1971 Oct 28;61(2):311–328. doi: 10.1016/0022-2836(71)90382-2. [DOI] [PubMed] [Google Scholar]
  6. KAISER A. D., HOGNESS D. S. The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg. J Mol Biol. 1960 Dec;2:392–415. doi: 10.1016/s0022-2836(60)80050-2. [DOI] [PubMed] [Google Scholar]
  7. Katzir N., Oppenheim A., Belfort M., Oppenheim A. B. Activation of the lambda int gene by the cii and ciii gene products. Virology. 1976 Oct 15;74(2):324–331. doi: 10.1016/0042-6822(76)90339-1. [DOI] [PubMed] [Google Scholar]
  8. Lang D., Mitani M. Simplified quantitative electron microscopy of biopolymers. Biopolymers. 1970;9(3):373–379. doi: 10.1002/bip.1970.360090310. [DOI] [PubMed] [Google Scholar]
  9. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oppenheim A. B. Internal inversion used to study regulation of the int gene in bacteriophage lambda. Nature. 1976 Jun 17;261(5561):615–616. doi: 10.1038/261615a0. [DOI] [PubMed] [Google Scholar]
  11. Schwartz S. A., Helinski D. R. Purification and characterization of colicin E1. J Biol Chem. 1971 Oct 25;246(20):6318–6327. [PubMed] [Google Scholar]
  12. Shimada K., Campbell A. Int-constitutive mutants of bacteriophage lambda. Proc Natl Acad Sci U S A. 1974 Jan;71(1):237–241. doi: 10.1073/pnas.71.1.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tanaka T., Weisblum B. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol. 1975 Jan;121(1):354–362. doi: 10.1128/jb.121.1.354-362.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thomas M., Cameron J. R., Davis R. W. Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4579–4583. doi: 10.1073/pnas.71.11.4579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
  16. Tikhomirova L. P., Solonin A. S., Ksenzenko V. N., Matvienko N. I. Construction of a hybrid col E1 plasmid carrying the gene for bacteriophage lambda repressor. Nucleic Acids Res. 1976 Oct;3(10):2485–2490. doi: 10.1093/nar/3.10.2485. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES