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. 1978 Jun;5(6):2211–2222. doi: 10.1093/nar/5.6.2211

Preparation and isolation of covalently closed circular rDNA molecules from DNA of Xenopus laevis.

L C Polito, S Spadari
PMCID: PMC342154  PMID: 673851

Abstract

We describe a method leading to the formation of closed circles of rDNA starting from total DNA of Xenopus laevis. Linear DNA molecules were digested with exonuclease 3 and self-annealed. Open circles were enriched and covalently closed by the simultaneous use of polynucleotide kinase, DNA polymerase and polynucleotide ligase. Closed circles of rDNA1 were shown to be alkali-resistant, to have higher density than linear molecules in cesium chloride density gradients containing ethydium bromide, and to have the sedimentation constant expected for a single repeat unit of rDNA comprehensive of its spacer.

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Selected References

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