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. 1978 Jul;5(7):2223–2232. doi: 10.1093/nar/5.7.2223

Biospecific fractionation matrices for sequence specific endonucleases

Jay George 1, Jack G Chirikjian 1
PMCID: PMC342159  PMID: 673852

Abstract

Fractionation of several type II specific restriction endonucleases was achieved by separation on two novel biospecific matrices. The matrices are pyran, a copolymer of divinyl ether of maleic anhydride, and Cibacron Blue F3GA, a blue dye commonly used for the calibration of molecular sieves. Both compounds are insolubilized by coupling to sepharose through a cyanogen bromide linkage and in their soluble form inhibit the restriction endonucleases which we have tested. These affinity matrices can be used to obtain restriction endonucleases from crude extracts after removal of nucleic acids. They have also proven to have a high capacity when used as subsequent steps in enzyme purification. Their additional advantage is the rapid development time and reusability of columns packed with the two matrices.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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