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. 1978 Jul;5(7):2557–2564. doi: 10.1093/nar/5.7.2557

Measurement of the differential melting profile of a promoter-containing fragment of T7 DNA by means of a microspectrophotometer.

M A Grachev, M P Perelroyzen
PMCID: PMC342185  PMID: 673861

Abstract

A double-beam microspectrophotometer with a 5 microliter cell has been used to study denaturation of DNA in an aqueous solution. This instrument enables measurement of high-resolution differential melting profiles simultaneously at several wavelengths with 0.5 to 1 microgram of DNA. Therefore it becomes possible to study nucleic acids which are difficult to obtain in large amounts. The techniques have been employed to measure the differential melting profiles of T7 DNA and of a fragment of this DNA 1000 base pairs long which contains the four early promoters.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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