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. 1978 Jul;5(7):2577–2586. doi: 10.1093/nar/5.7.2577

Synthesis of DNA in permeabilized cells of Kluyveromyces lactis.

G Badaracco, M Laquidara, G Cassani
PMCID: PMC342187  PMID: 673862

Abstract

Kluyveromyces lactis cells permeabilized with nystatin, though no longer viable, were able to incorporate 3H-dATP into DNA. Maximum rate of synthesis was obtained when all four deoxyribonucleoside triphosphates were present. For prolonged incorporation of 3H-dATP into DNA rATP or phosphoenolpyruvate were of absolute requirement. DNA synthesis was inhibited by p-chloromercuribenzoate, N-ethylmaleimide, nalidixate, ethidium bromide and distamycin A. The density of DNA synthesized in permeabilized cells grown on non-fermentable and fermentable carbon sources was analyzed on CsCl gradients in the presence or absence of distamycin A. The DNA synthesized by permeabilized cells previously grown on glycerol was essentially mitochondrial DNA; nuclear DNA (30% of total) was also synthesized by cells previously grown on glucose.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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