Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1978 Jul;5(7):2607–2616. doi: 10.1093/nar/5.7.2607

Comparisons of the fidelity of transcription of RNA polymerase I and II following N-hydroxy-2-acetylaminofluorene treatment.

R I Glazer
PMCID: PMC342189  PMID: 353743

Abstract

Using hepatic RNA polymerase I and II from either normal or N-2-hydroxy-2-acetylaminofluorene (N-OH-AAF)-treated rats or E. coli RNA polymerase, the degree of misincorporation of noncomplementary nucleotides was assessed with the synthetic templates, poly(dG-dC).poly(dG-dC) and poly (dA-dT).poly(dA-dT). The predominant base-pair transformation that was transcribed in the presence of Mg++ or Mn++ by RNA polymerase I from control or N-OH-AAF-treated animals or by E. coli RNA polymerase with poly(dG-dC).poly(dG-dC) as template was the transversion, dG-rC to dG-rA; however, transcription in the presence of Mg++ by RNA polymerase II from carcinogen-treated animals showed a statistically greater degree of the base-pair transformation, dG-rC to dG-rU. In contrast, RNA polymerase I and II from control or N-OH-AAF-treated animals transcribed the base-pair transformation, dA-rU to dA-rG, dA-rU to dA-rC and dA-rU to dA-rA to equal extents with poly(dA-dT).poly(dA-dT) as template. E. coli RNA polymerase transcribed the latter template to produce only the transversion, dA-rU to dA-rG. These results suggest that RNA polymerases are capable of miscopying synthetic DNA templates. The consequences of base-pair transformations on the fidelity of transcription after carcinogen treatment is discussed.

Full text

PDF
2607

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akao M., Kuroda K., Tsutsui Y., Kanisawa M., Miyaki K. Effect of nitrofurans antagonistic to 3'-methyl-4-dimethylaminoazobenzene in hepatocarcinogenesis and RNA polymerase activity of liver cell nuclei in rats. Cancer Res. 1974 Aug;34(8):1843–1850. [PubMed] [Google Scholar]
  2. Dezélée S., Sentenac A., Fromageot P. Role of deoxyribonucleic acid-ribonucleic acid hybrids in eukaryotes. Synthetic ribo- and deoxyribopolynucleotides as template for yeast ribonucleic acid polymerase B (or II). J Biol Chem. 1974 Sep 25;249(18):5978–5983. [PubMed] [Google Scholar]
  3. Glazer R. I. Alterations in the transcriptional capacity of hepatic DNA-dependent RNA polymerase I and II by N-hydroxy-2-acetylaminofluorene. Cancer Res. 1976 Jul;36(7 Pt 1):2282–2289. [PubMed] [Google Scholar]
  4. Glazer R. I., Glass L. E., Menger F. M. Modification of hepatic ribonucleic acid polymerase activities by N-hydroxy-2-acetlaminofluorene and N-acetoxy-2-acetylaminofluorene. Mol Pharmacol. 1975 Jan;11(1):36–43. [PubMed] [Google Scholar]
  5. Glazer R. I. Inhibition by N-hydroxy-2-acetylaminofluorene of incorporation of (5-3H)orotic acid into RNA of two classes of nuclei from normal and regenerating liver. Biochim Biophys Acta. 1974 Sep 13;361(3):361–366. doi: 10.1016/0005-2787(74)90379-7. [DOI] [PubMed] [Google Scholar]
  6. Glazer R. I., Nutter R. C., Glass L. E., Menger F. M. 2-Acetylaminofluorene and N-hydroxy-2-acetylaminofluorene inhibition of incorporation of orotic acid-5-3H into nuclear ribosomal and heterogeneous RNA in normal and regenerating liver. Cancer Res. 1974 Oct;34(10):2451–2458. [PubMed] [Google Scholar]
  7. Grunberger G., Yu F. L., Grunberger D., Feigelson P. Mechanism of N-hydroxy-2-acetylaminofluorene inhibition of rat hepatic ribonucleic acid synthesis. J Biol Chem. 1973 Sep 25;248(18):6278–6281. [PubMed] [Google Scholar]
  8. Herzog J., Farber J. L. Inhibition of rat liver RNA polymerases by action of the methylating agents dimethylnitrosamine in vivo and methyl methanesulfonate in vitro. Cancer Res. 1976 May;36(5):1761–1770. [PubMed] [Google Scholar]
  9. Herzog J., Serroni A., Briesmeister B. A., Farber J. L. N-hydroxy-2-acetylaminofluorene inhibition of rat live RNA polymerases. Cancer Res. 1975 Aug;35(8):2138–2144. [PubMed] [Google Scholar]
  10. Loeb L. A., Springgate C. F., Battula N. Errors in DNA replication as a basis of malignant changes. Cancer Res. 1974 Sep;34(9):2311–2321. [PubMed] [Google Scholar]
  11. Mizutani S., Temin H. M. Incorporation of noncomplementary nucleotides at high frequencies by ribodeoxyvirus DNA polymerases and Escherichia coli DNA polymerase I. Biochemistry. 1976 Apr 6;15(7):1510–1516. doi: 10.1021/bi00652a023. [DOI] [PubMed] [Google Scholar]
  12. POIRIER L. A., MILLER J. A., MILLER E. C. The N- and ring-hydroxylation of 2-acetylaminofluorene and the failure to detect N-acetylation of 2-aminofluorene in the dog. Cancer Res. 1963 Jun;23:790–800. [PubMed] [Google Scholar]
  13. Roeder R. G., Rutter W. J. Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms. Nature. 1969 Oct 18;224(5216):234–237. doi: 10.1038/224234a0. [DOI] [PubMed] [Google Scholar]
  14. Rose K. M., Ruch P. A., Jacob S. T. Mechanism of inhibition of RNA polymerase II and poly(adenylic acid) polymerase by the O-n-octyloxime of 3-formylrifamycin SV. Biochemistry. 1975 Aug 12;14(16):3598–3604. doi: 10.1021/bi00687a013. [DOI] [PubMed] [Google Scholar]
  15. Saunders F. C., Barker E. A., Smuckler E. A. Selective inhibition of nucleoplasmic rat liver DNA-dependent RNA polymerase by aflatoxin B 1 . Cancer Res. 1972 Nov;32(11):2487–2494. [PubMed] [Google Scholar]
  16. Sirover M. A., Loeb L. A. Erroneous base-pairing induced by a chemical carcinogen during DNA synthesis. Nature. 1974 Nov 29;252(5482):414–416. doi: 10.1038/252414a0. [DOI] [PubMed] [Google Scholar]
  17. Springgate C. F., Loeb L. A. On the fidelity of transcription by Escherichia coli ribonucleic acid polymerase. J Mol Biol. 1975 Oct 5;97(4):577–591. doi: 10.1016/s0022-2836(75)80060-x. [DOI] [PubMed] [Google Scholar]
  18. Temin H. M. The DNA provirus hypothesis. Science. 1976 Jun 11;192(4244):1075–1080. doi: 10.1126/science.58444. [DOI] [PubMed] [Google Scholar]
  19. Zieve F. J. Inhibition of rat liver ribonucleic acid polymerase by the carcinogen N-hydroxy-2-fluorenylacetamide. J Biol Chem. 1972 Sep 25;247(18):5987–5995. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES