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. 1978 Aug;5(8):2721–2728. doi: 10.1093/nar/5.8.2721

pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

J G Sutcliffe
PMCID: PMC342202  PMID: 358147

Abstract

I have derived a complete restriction map of pBR322 from the total nucleotide sequence of the plasmid. Most of the restriction sites also have been demonstrated empirically. The exact sizes of all restriction fragments and the relative positions of the cuts are presented. These fragments can serve as accurate DNA size markers from small pieces up to the 4362 base pair length of pBR322. Inserts cloned in this vector may be characterized easily using this data.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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