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. 1978 Aug;5(8):2969–2977. doi: 10.1093/nar/5.8.2969

Ultraviolet resonance Raman study of DNA and of its interaction with actinomycin D.

L Chinsky, P Y Turpin
PMCID: PMC342219  PMID: 567800

Abstract

The DNA-Actinomycin D interaction has been studied by resonance Raman effect using DNA as chromophore. First, the resonance Raman spectra of DNA obtained with a U.V. excitation at wavelengths of 300 nm and 280 nm are presented. The main Raman hands are assigned to the convenient nucleic bases by comparison with the spectra of mononucleotides obtained under the same experimental conditions. In particular, with a 300 nm excitation, the 1582 cm-1 line is provided by adenine, while the 1492 cm-1 one is almost exclusively due to guanine. Then, the DNA-Actinomycin D complex has been studied: the line enhancements and the specificity of the resonance permits the displaying of the DNA spectrum free of any contribution of Actinomycin. The interaction provides a large intensity decrease of the 1492 cm-1 guanine line: this is a direct consequence of the orbital overlapping of the guanine 2-aminogroup with the ring nitrogen of Actinomycin in the DNA-Actinomycin pi complex.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blazej D. C., Peticolas W. L. Ultraviolet resonant Raman spectroscopy of nucleic acid components. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2639–2643. doi: 10.1073/pnas.74.7.2639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chinsky L., Turpin P. Y., Duquesne M., Brahms J. Structral investigation of poly d (BrU-A) by ultraviolet resonance Raman spectroscopy. Biochem Biophys Res Commun. 1977 Apr 11;75(3):766–771. doi: 10.1016/0006-291x(77)91538-8. [DOI] [PubMed] [Google Scholar]
  3. Chinsky L., Turpin P. Y., Duquesne M. Resonance Raman study of actinomycin D interaction with DNA and its models. Biochem Biophys Res Commun. 1975 Aug 18;65(4):1440–1446. doi: 10.1016/s0006-291x(75)80390-1. [DOI] [PubMed] [Google Scholar]
  4. Chinsky L., Turpin P. Y. Fluorescence of actinomycin D and its DNA complex. Biochim Biophys Acta. 1977 Mar 2;475(1):54–63. doi: 10.1016/0005-2787(77)90338-0. [DOI] [PubMed] [Google Scholar]
  5. GELLERT M., SMITH C. E., NEVILLE D., FELSENFELD G. ACTINOMYCIN BINDING TO DNA: MECHANISM AND SPECIFICITY. J Mol Biol. 1965 Mar;11:445–457. doi: 10.1016/s0022-2836(65)80001-8. [DOI] [PubMed] [Google Scholar]
  6. Mansy S., Peticolas W. L. Detection of the sites of alkylation in DNA and polynucleotides by laser Raman spectroscopy. Biochemistry. 1976 Jun 15;15(12):2650–2655. doi: 10.1021/bi00657a026. [DOI] [PubMed] [Google Scholar]
  7. Müller W., Crothers D. M. Studies of the binding of actinomycin and related compounds to DNA. J Mol Biol. 1968 Jul 28;35(2):251–290. doi: 10.1016/s0022-2836(68)80024-5. [DOI] [PubMed] [Google Scholar]
  8. Small E. W., Peticolas W. L. Conformational dependence of the Raman scattering intensities from polynucleotides. 3. Order-disorder changes in helical structures. Biopolymers. 1971;10(8):1377–1418. doi: 10.1002/bip.360100811. [DOI] [PubMed] [Google Scholar]
  9. Sobell H. M., Jain S. C. Stereochemistry of actinomycin binding to DNA. II. Detailed molecular model of actinomycin-DNA complex and its implications. J Mol Biol. 1972 Jul 14;68(1):21–34. doi: 10.1016/0022-2836(72)90259-8. [DOI] [PubMed] [Google Scholar]
  10. Wells R. D., Larson J. E. Studies on the binding of actinomycin D to DNA and DNA model polymers. J Mol Biol. 1970 Apr 28;49(2):319–342. doi: 10.1016/0022-2836(70)90248-2. [DOI] [PubMed] [Google Scholar]

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